The mitochondrial gene orfH79 plays a critical role in impairing both male gametophyte development and root growth in CMS-Honglian rice

BMC Plant Biology

Research Article

Chaofeng Hu¹  Yingguo Zhu¹  Jing Yang¹  Shaoqing Li¹  Kun Wang¹  Ting Wang¹  Jiping Tong¹  Xiaojue Peng²  Youlin Zhu³ 

[1] Key Laboratory of MOE for Plant Developmental Biology, College of Life Science, Wuhan University, 430072, Wuhan, PR, China;Key Laboratory of MOE for Plant Developmental Biology, College of Life Science, Wuhan University, 430072, Wuhan, PR, China;Key Laboratory of Molecular Biology and Gene Engineering, College of life Science, Nanchang University, 330047, Nanchang, PR, China;Key Laboratory of Molecular Biology and Gene Engineering, College of life Science, Nanchang University, 330047, Nanchang, PR, China;

关键词: Reactive Oxygen Species;    Transgenic Plant;    Mitochondrial Membrane Potential;    Male Sterility;    Cytoplasmic Male Sterility;   

DOI  :  10.1186/1471-2229-10-125

 received in 2009-11-12, accepted in 2010-06-24,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundCytoplasmic male sterility (CMS) has often been associated with abnormal mitochondrial open reading frames. The mitochondrial gene orfH79 is a candidate gene for causing the CMS trait in CMS-Honglian (CMS-HL) rice. However, whether the orfH79 expression can actually induce CMS in rice remains unclear.ResultsWestern blot analysis revealed that the ORFH79 protein is mainly present in mitochondria of CMS-HL rice and is absent in the fertile line. To investigate the function of ORFH79 protein in mitochondria, this gene was fused to a mitochondrial transit peptide sequence and used to transform wild type rice, where its expression induced the gametophytic male sterile phenotype. In addition, excessive accumulation of reactive oxygen species (ROS) in the microspore, a reduced ATP/ADP ratio, decreased mitochondrial membrane potential and a lower respiration rate in the transgenic plants were found to be similar to those in CMS-HL rice. Moreover, retarded growth of primary and lateral roots accompanied by abnormal accumulation of ROS in the root tip was observed in both transgenic rice and CMS-HL rice (YTA).ConclusionThese results suggest that the expression of orfH79 in mitochondria impairs mitochondrial function, which affects the development of both male gametophytes and the roots of CMS-HL rice.

【 授权许可】

Unknown   
© Peng et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.