Anti-CDC25B autoantibody predicts poor prognosis in patients with advanced esophageal squamous cell carcinoma

Journal of Translational Medicine

Research

Mu-sheng Zeng¹  Li-hua Xu¹  Man-Zhi Li¹  Wan-li Liu²  Jun-ye Wang³  Jun Dong⁴  Bo-hang Zeng⁴ 

[1] Department of Experimental Research, Sun Yat-sen University Cancer Center, Guangzhou, China;State Key Laboratory of Oncology in South China and Department of Thoracic, Sun Yat-sen University Cancer Center, Guangzhou, China;State Key Laboratory of Oncology in South China and Department of Thoracic, Sun Yat-sen University Cancer Center, Guangzhou, China;Department of Clinical Laboratory Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China;State Key Laboratory of Oncology in South China and Department of Thoracic, Sun Yat-sen University Cancer Center, Guangzhou, China;Department of Thoracic, Sun Yat-sen University Cancer Center, Guangzhou, China;The Second Affiliated Hospital of Guangzhou Medical University, Gyangzhou, China;

关键词: Esophageal Squamous Cell Carcinoma;    Esophageal Squamous Cell Carcinoma Patient;    Esophageal Squamous Cell Carcinoma Cell Line;    Advanced Esophageal Squamous Cell Carcinoma;    Primary Esophageal Squamous Cell Carcinoma;   

DOI  :  10.1186/1479-5876-8-81

 received in 2010-03-08, accepted in 2010-09-03,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundThe oncogene CDC25B phosphatase plays an important role in cancer cell growth. We have recently reported that patients with esophageal squamous cell carcinoma (ESCC) have significantly higher serum levels of CDC25B autoantibodies (CDC25B-Abs) than both healthy individuals and patients with other types of cancer; however, the potential diagnostic or prognostic significance of CDC25B-Abs is not clear. The aim of this study is to evaluate the clinical significance of serum CDC25B-Abs in patients with ESCC.MethodsCDC25B autoantibodies were measured in sera from both 134 patients with primary ESCC and 134 healthy controls using a reverse capture enzyme-linked immunosorbent assay (ELISA) in which anti-CDC25B antibodies bound CDC25B antigen purified from Eca-109 ESCC tumor cells. The clinicopathologic significance of CDC25B serum autoantibodies was compared to that of the tumor markers carcinoembryonic antigen (CEA), squamous cell carcinoma antigen (SCC-Ag) and cytokeratin 19 fragment antigen 21-1(CYFRA21-1).ResultsHigher levels of CDC25B autoantibodies were present in sera from patients with ESCC (A450 = 0.917, SD = 0.473) than in sera from healthy control subjects (A450 = 0.378, SD = 0.262, P < 0.001). The area under the receiver operating characteristic (ROC) curve for CDC25B-Abs was 0.870 (95% CI: 0.835-0.920). The sensitivity and specificity of CDC25B-Abs for detection of ESCC were 56.7% and 91.0%, respectively, when CDC25-Abs-positive samples were defined as those with an A450 greater than the cut-off value of 0.725. Relatively few patients tested positive for the tumor markers CEA, SCC-Ag and CYFRA21-1 (13.4%, 17.2%, and 32.1%, respectively). A significantly higher number of patients with ESCC tested positive for a combination of CEA, SCC, CYFRA21-1 and CDC25B-Abs (64.2%) than for a combination of CEA, SCC-Ag and CYFRA21-1 (41.0%, P < 0.001). The concentration of CDC25B autoantibodies in serum was significantly correlated with tumor stage (P < 0.001). Although examination of the total patient pool showed no obvious relationship between CDC25B autoantibodies and overall survival, in the subgroup of patients with stage III-IV tumors, the cumulative five-year survival rate of CDC25B-seropositive patients was 6.7%, while that of CDC25B-seronegative patients was 43.4% (P = 0.001, log-rank). In the N1 subgroup, the cumulative five-year survival rate of CDC25B-seropositive patients was 13.6%, while that of CDC25B-seronegative patients was 54.5% (P = 0.040, log-rank).ConclusionsDetection of serum CDC25B-Abs is superior to detection of the tumor markers CEA, SCC-Ag and CYFRA21-1 for diagnosis of ESCC, and CDC25B-Abs are a potential prognostic serological marker for advanced ESCC.

【 授权许可】

Unknown   
© Dong et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.