Genome-wide association mapping and Identification of candidate genes for fatty acid composition in Brassica napus L. using SNP markers

BMC Genomics

Research Article

Lijuan Wei¹  Huiyan Zhao¹  Shimeng Li¹  Jiana Li¹  Ledong Jia¹  Ying Liang¹  Kun Lu¹  Cunmin Qu¹  Xinfu Xu¹  Rui Wang¹  Fuyou Fu² 

[1] Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University, 400716, Chongqing, China;Engineering Research Center of South Upland Agriculture of Ministry of Education, Southwest University, Beibei, 400716, Chongqing, China;Department of Botany and Plant Pathology, Purdue University, 915 W. State Street, 47907-2054, West Lafayette, IN, USA;

关键词: Association mapping;    Brassica napus;    Candidate gene;    Fatty acid components;    Single Nucleotide Polymorphism (SNP);   

DOI  :  10.1186/s12864-017-3607-8

 received in 2016-08-03, accepted in 2017-03-03,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundB. napus (oilseed) is an important source of edible vegetable oil, and its nutritional and economic value is determined by its fatty acid composition and content.ResultsUsing the Brassica 60 K SNP array, we performed a genome-wide association study of fatty acid composition in a population of 520 genetically diverse oilseed accessions. Using the PCA + K model in TASSEL 5.2.1, we identified 62 genomic regions that were significantly associated with the composition of seven fatty acids, and five consensus regions that mapped to the A2, A8, A9, C1, and C3 chromosomes, respectively, of the Brassica napus Darmor-bzh genome. We then identified 24 orthologs of the functional candidate genes involved in fatty acid biosynthesis, excluding BnaA.FAE1 and BnaC.FAE1 on the A8 and C3 homologous genome blocks, which are known to have critical roles in the fatty acid biosynthesis pathway, and potential orthologs of these genes (e.g., LACS9, KCR1, FAB1, LPAT4, KCS17, CER4, TT16, and ACBP5).ConclusionsOur results demonstrate the power of association mapping in identifying genes of interest in B. napus and provide insight into the genetic basis of fatty acid biosynthesis in B. napus. Furthermore, our findings may facilitate marker-based breeding efforts aimed at improving fatty acid composition and quality in B. napus.

【 授权许可】

CC BY   
© The Author(s). 2017