Genetic diversity and molecular typing of Listeria monocytogenes in China

BMC Microbiology

Research Article

Renfa Zhu¹  Yonglu Wang²  Ruiting Lan³  Yiting Wang⁴  Changyun Ye⁴  Zhigang Cui⁴  Dong Jin⁴  Yan Wang⁴  Zhenchuan Li⁴  Ailan Zhao⁴  Jianguo Xu⁴ 

[1] Foshan Municipal Bureau of Agriculture, Guangdong Province, China;Maanshan Center for Disease Control and Prevention, Anhui Province, China;School of Biotechnology and Biomolecular Sciences, University of New South Wales, 2052, Sydney, NSW, Australia;State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, China CDC, Changbai Road 155, Changping, Beijing, China;

关键词: Clonal Complex;    Unweighted Pair Group Method With Arithmetic;    Listeriosis;    MLST Scheme;    MLST Data;   

DOI  :  10.1186/1471-2180-12-119

 received in 2012-02-02, accepted in 2012-06-14,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundListeria monocytogenes can cause invasive diseases in humans and farm animals and is frequently isolated from dairy products and poultry. Listeriosis is uncommon in China but L. monocytogenes has been isolated from foods and food processing environments in China. However little is known of genetic diversity of Chinese L. monocytogenes isolates and their relationships with global isolates.ResultsTwo hundred and twelve isolates of L. monocytogenes from food sources from 12 provinces/cities in China were analysed by serotyping, Pulsed Field Gel Electrophoresis (PFGE) and Multi-locus Sequence Typing (MLST). The predominant serotypes are 1/2a, 1/2b and 1/2c accounting for 90.1% of the isolates. PFGE divided the isolates into 61 pulse types (PTs). Twenty nine PTs were represented by more than one isolates with PT GX6A16.0004 containing the most number of isolates. MLST differentiated the isolates into 36 STs, among which 15 were novel. The 3 most common STs were ST9 (29.1%), ST8 (10.7%) and ST87 (9.2%), accounting for 49.0% of the isolates.ConclusionsSTs prevalent in other parts of the world are also prevalent in China including 7 STs (ST1-ST3, ST5, ST6, ST8, ST9) which caused maternal fetal infections or outbreaks, suggesting that these STs potentially can also cause severe human infections or outbreaks in China. Surveillance of these STs will provide important information for prevention of listeriosis. This study also enhances our understanding of genetic diversity of L. monocytogenes in China.

【 授权许可】

Unknown   
© Wang et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.