Pinewood nematode-associated bacteria contribute to oxidative stress resistance of Bursaphelenchus xylophilus

BMC Microbiology

Research Article

Yoriko Ikuyo¹  Koichi Hasegawa¹  Cláudia S L Vicente²  Manuel Mota³ 

[1] Department of Environmental Biology, College of Bioscience & Biotechnology, Chubu University, 1200 Matsumoto, 487-8501, Kasugai, Aichi, Japan;Department of Environmental Biology, College of Bioscience & Biotechnology, Chubu University, 1200 Matsumoto, 487-8501, Kasugai, Aichi, Japan;ICAAM - Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Departamento de Biologia, Universidade de Évora, Núcleo da Mitra, Ap. 94, 7002-554, Évora, Portugal;ICAAM - Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Departamento de Biologia, Universidade de Évora, Núcleo da Mitra, Ap. 94, 7002-554, Évora, Portugal;INIAV/Unidade Estratégica de Investigação e Serviços de Sistemas Agrários e Florestais e Sanidade Vegetal Av. da República, 2784-159, Quinta do Marquês, Oeiras, Portugal;

关键词: Bursaphelenchus xylophilus;    Catalase;    Oxidative stress;    Pine wilt disease;   

DOI  :  10.1186/1471-2180-13-299

 received in 2013-08-16, accepted in 2013-12-18,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundPine wilt disease (PWD) caused by the pinewood nematode Bursaphelenchus xylophilus is one of the most serious forest diseases in the world. The role of B. xylophilus-associated bacteria in PWD and their interaction with the nematode, have recently been under substantial investigation. Several studies report a potential contribution of the bacteria for the PWD development, either as a helper to enhance the pathogenicity of the nematode or as a pathogenic agent expressing interesting traits related to lifestyle host-adaptation.ResultsWe investigated the nematode-bacteria interaction under a severe oxidative stress (OS) condition using a pro-oxidant hydrogen peroxide and explored the adhesion ability of these bacteria to the cuticle surface of the nematodes. Our results clearly demonstrated a beneficial effect of the Serratia spp. (isolates LCN-4, LCN-16 and PWN-146) to B. xylophilus under the OS condition. Serratia spp. was found to be extremely OS-resistant, and promote survival of B. xylophilus and down-regulate two B. xylophilus catalase genes (Bxy-ctl-1 and Bxy-ctl-2). In addition, we show that the virulent isolate (Ka4) of B. xylophilus survives better than the avirulent (C14-5) isolate under the OS condition. The bacterial effect was transverse for both B. xylophilus isolates. We could not observe a strong and specific adhesion of these bacteria on the B. xylophilus cuticle surface.ConclusionsWe report, for the first time, that B. xylophilus associated bacteria may assist the nematode opportunistically in the disease, and that a virulent B. xylophilus isolate displayed a higher tolerance towards the OS conditions than an avirulent isolate.

【 授权许可】

Unknown   
© Vicente et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.