Chicken model of steroid-induced bone marrow adipogenesis using proteome analysis: a preliminary study

Proteome Science

Research

Yun Ho Lin¹  Chia Chun Chen²  Wing P Chan³  Tzong Fu Kuo⁴  Ching Yu Lin⁵  Way Neng Lin⁶  Sing Chung Li⁶ 

[1] Department of Pathology, School of Medicine, Taipei Medical University, 110, Taipei, Taiwan, Republic of China;Department of Radiology, School of Medicine, Taipei Medical University, 110, Taipei, Taiwan, Republic of China;Department of Radiology, School of Medicine, Taipei Medical University, 110, Taipei, Taiwan, Republic of China;Department of Radiology, Taipei Medical University-Wan Fang Hospital, 116, Taipei, Taiwan, Republic of China;Graduate Institute of Veterinary Medicine, National Taiwan University, 110, Taipei, Taiwan, Republic of China;School of Medical Technology and Biotechnology, Taipei Medical University, 110, Taipei, Taiwan, Republic of China;School of Nutrition and Health Sciences, Taipei Medical University, 110, Taipei, Taiwan, Republic of China;

关键词: Osteonecrosis;    Bone Marrow Aspiration;    Trabecular Bone Loss;    Femoral Bone Marrow;    Chicken Model;   

DOI  :  10.1186/1477-5956-8-47

 received in 2010-05-15, accepted in 2010-09-14,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundSteroid-induced adipogenesis increases fat-cell volume and pressure in bone marrow. This may be a contributing factor in some forms of osteonecrosis. In this observational study, we aimed to determine the protein expression relating to steroid-induced adipogenesis of femoral bone marrow with use of a chicken model. We compared the histologic features of the femoral marrow of eight methylprednisolone (MP)-treated chickens with those of three control chickens and assessed differential proteins with 2-dimensional gel electrophoresis and differential proteins were identified by MALDI-TOF MS.ResultsOne MP-induced chicken died of overdose anesthesia. Methylprednisolone-induced proliferation of adipose tissue and new bone formation were found on histologic examination. In our study, 13 proteins in the control and MP-induced groups were differently expressed and nine protein spots showed marked threefold downregulation after 19 weeks of MP treatment. These were serum amyloid P-component precursor, zinc finger protein 28, endothelial zinc finger protein 71, T-box transcription factor 3, cyclin-dependent kinase inhibitor 1, myosin 1D, dimethylaniline monooxygenase, and two uncharacterized proteins.ConclusionsProteomic profiling can be a useful dynamic approach for detecting protein expression in MP-induced adipogenesis of the femur in chickens.

【 授权许可】

Unknown   
© Li et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.