The influence of the size and aspect ratio of anisotropic, porous CaCO3 particles on their uptake by cells

Journal of Nanobiotechnology

Research

Helmuth Möhwald¹  Andre G Skirtach²  Wolfgang J Parak³  Joanna Rejman³  Mikhail V Zyuzin³  Susana Carregal-Romero³  Alexey Yashchenok⁴  Bogdan Parakhonskiy⁵ 

[1] Department of Interfaces, Max-Planck Institute of Colloids and Interfaces, Potsdam, Germany;Department of Interfaces, Max-Planck Institute of Colloids and Interfaces, Potsdam, Germany;NanoBio-Photonics, Ghent University, Ghent, Belgium;Department of Molecular Biotechnology, Ghent University, Ghent, Belgium;Fachbereich Physik, Philipps University of Marburg, Marburg, Germany;Institute of Nanostructures and Biosystems, Saratov State University, Saratov, Russia;Department of Interfaces, Max-Planck Institute of Colloids and Interfaces, Potsdam, Germany;Shubnikov Institute of Crystallography, Russian Academy of Science, Moscow, Russia;Institute of Nanostructures and Biosystems, Saratov State University, Saratov, Russia;

关键词: Calcium carbonate;    Anisotropic;    Uptake;    Cells;    Internalisation;   

DOI  :  10.1186/s12951-015-0111-7

 received in 2015-04-16, accepted in 2015-07-28,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundRecent reports highlighting the role of particle geometry have suggested that anisotropy can affect the rate and the pathway of particle uptake by cells. Therefore, we investigate the internalization by cells of porous calcium carbonate particles with different shapes and anisotropies.ResultsWe report here on a new method of the synthesis of polyelectrolyte coated calcium carbonate particles whose geometry was controlled by varying the mixing speed and time, pH value of the reaction solution, and ratio of the interacting salts used for particle formation. Uptake of spherical, cuboidal, ellipsoidal (with two different sizes) polyelectrolyte coated calcium carbonate particles was studied in cervical carcinoma cells. Quantitative data were obtained from the analysis of confocal laser scanning microscopy images.ConclusionsOur results indicate that the number of internalized calcium carbonate particles depends on the aspect ratio of the particle, whereby elongated particles (higher aspect ratio) are internalized with a higher frequency than more spherical particles (lower aspect ratio). The total volume of internalized particles scales with the volume of the individual particles, in case equal amount of particles were added per cell.

【 授权许可】

CC BY   
© Parakhonskiy et al. 2015

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