The effect of oxidized low-density lipoprotein combined with adriamycin on the proliferation of Eca-109 cell line

Lipids in Health and Disease

Research

Mei Shu Wang¹  Ping Zhi Wang¹  Qing Hui Li²  Tao Yu Diao²  Jia Cui²  Hao Li³  Qing D Li³ 

[1] College of Public Health, Shandong University, Jinan, P.R. China;Institutes of Basic Medicine, Shandong Academy of Medical Sciences Jinan, P.R. China;Qilu Hospital, Shandong University, Jinan, P.R. China;

关键词: Esophagueal squamous cell line;    low-density lipoprotein;    adriamycin;    apoptosis;    gene;    protein;    expression;   

DOI  :  10.1186/1476-511X-10-108

 received in 2011-05-12, accepted in 2011-06-29,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundThe purpose of this study was to identify the affect on the proliferation Eca-109 cells treated with oxidized low-density lipoprotein (ox-LDL) combined with adriamycin (ADM).MethodsEca-109 cell were cultured in the presence of oxLDL/ADM, and cell proliferation tested by MTT and cell apoptosis was monitored by the proportion of apoptosis and cell cycle by flow cytomester. We simultaneously evaluated the level of associated- apoptosis Bcl-2, Bax, and Caspase-3 gene mRNA and protein.ResultsOxLDL were cytotoxic and activate apoptosis. OxLDL combined with ADM significant enhanced the proportion rate of apoptosis on a time and dose dependency. The expressions of the inhibiting apoptosis Bcl-2 gene mRNA and protein were down regulated, whereas, the expressions of the promoting apoptosis Bax, and Caspase-3 genes mRNA and protein were up regulation.ConclusionThese results suggested that oxLDL have cytotoxicity and activate apoptosis on the Eca-109 cells. OxLDL combined with ADM have a synergistic effect on the apoptosis induced Eca-109 cells. Furthermore, oxLDL may contribute to the improvement of clinical chemotherapy of cancer need to make further investigation.

【 授权许可】

CC BY   
© Li et al; licensee BioMed Central Ltd. 2011