Recombinant domains III of Tick-Borne Encephalitis Virus envelope protein in combination with dextran and CpGs induce immune response and partial protectiveness against TBE virus infection in mice

BMC Infectious Diseases

Research Article

Galina G. Karganova¹  Larissa V. Gmyl¹  Ivan S. Kholodilov¹  Liubov I. Kozlovskaya¹  Artem P. Tkachuk²  Vladimir P. Gudov²  Darya M. Savina²  Zoya M. Galushkina²  Alexander M. Lyaschuk²  Tatyana M. Grunina²  Mikhail S. Bartov²  Olga A. Gra²  Alexander L. Gintsburg²  Olga V. Sergienko³  Vladimir G. Lunin³  Anna S. Ershova⁴  Anna S. Karyagina⁴ 

[1] Chumakov Institute of poliomyelitis and viral encephalitides, 142782, Moscow, Russia;Gamaleya Center of Epidemiology and Microbiology, 123098, Moscow, Russia;Gamaleya Center of Epidemiology and Microbiology, 123098, Moscow, Russia;Institute of Agricultural Biotechnology, 127550, Moscow, Russia;Gamaleya Center of Epidemiology and Microbiology, 123098, Moscow, Russia;Institute of Agricultural Biotechnology, 127550, Moscow, Russia;Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992, Moscow, Russia;

关键词: Tick-borne encephalitis virus;    Envelope protein;    Domain III;    Dextran-binding domain;    CpG oligonucleotides;   

DOI  :  10.1186/s12879-016-1884-5

 received in 2016-02-29, accepted in 2016-10-01,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundE protein of tick-borne encephalitis virus (TBEV) and other flaviviruses is located on the surface of the viral particle. Domain III of this protein seems to be a promising component of subunit vaccines for prophylaxis of TBE and kits for diagnostics of TBEV.MethodsThree variants of recombinant TBEV E protein domain III of European, Siberian and Far Eastern subtypes fused with dextran-binding domain of Leuconostoc citreum KM20 were expressed in E. coli and purified. The native structure of domain III was confirmed by ELISA antibody kit and sera of patients with tick-borne encephalitis. Immunogenic and protective properties of the preparation comprising these recombinant proteins immobilized on a dextran carrier with CpG oligonucleotides as an adjuvant were investigated on the mice model.ResultsAll 3 variants of recombinant proteins immobilized on dextran demonstrate specific interaction with antibodies from the sera of TBE patients. Thus, constructed recombinant proteins seem to be promising for TBE diagnostics. The formulation comprising the 3 variants of recombinant antigens immobilized on dextran and CpG oligonucleotides, induces the production of neutralizing antibodies against TBEV of different subtypes and demonstrates partial protectivity against TBEV infection.ConclusionsStudied proteins interact with the sera of TBE patients, and, in combination with dextran and CPGs, demonstrate immunogenicity and limited protectivity on mice compared with reference “Tick-E-Vac” vaccine.

【 授权许可】

CC BY   
© The Author(s). 2016