期刊论文详细信息
BMC Genomics
Oral microbial community typing of caries and pigment in primary dentition
Research Article
Yu Fu1  Yanhui Li2  Yanhong Li2  Juan Liu2  Qing Zhou2  Bo Liu2  Cheng-Gang Zou3  Zhigang Zhang4 
[1] Department of Dermatology, Beijing Hospital, 100730, Beijing, China;Department of Preventive & Pediatric Dentistry, The Affiliated Stomatology Hospital of Kunming Medical University, 650100, Kunming, Yunnan, China;State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, 650091, Kunming, Yunnan, China;State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, 650223, Kunming, China;
关键词: Saliva;    Supragingival plaque;    Pigment;    Caries;    Oral microbiota;   
DOI  :  10.1186/s12864-016-2891-z
 received in 2016-04-12, accepted in 2016-07-06,  发布年份 2016
来源: Springer
PDF
【 摘 要 】

BackgroundBlack extrinsic discoloration in primary dentition is a common clinical and aesthetic problem that can co-occur with dental caries, the most common oral diseases in childhood. Although the role of bacteria in the formation of pigment and caries in primary dentition is important, their basic features still remain a further mystery.MethodsUsing targeted sequencing of the V1-V3 hypervariable regions of bacterial 16S ribosomal RNA (rRNA) genes, we obtained a dataset consisting of 831,381 sequences from 111 saliva samples and 110 supragingival plaque samples from 40 patients with pigment (black extrinsic stain), 20 with caries (obvious decay), and 25 with both pigment and caries and from 26 healthy individuals. We applied a Dirichlet multinomial mixture (DMM)-based community typing approach to investigate oral microbial community types.ResultsOur results revealed significant structural segregation of microbial communities, as indicated by the identification of two plaque community types (A and B) and three saliva community types (C-E). We found that the independent occurrence of the two plaque community types, A and B, was potentially associated with our oral diseases of interest. For type A, three co-occurring bacterial genus pairs could separately play a potential role in the formation of pigment (Leptotrichia and Fusobacterium), caries (unclassified Gemellales and Granulicatella), and mixed caries and pigment (Streptococcus and Mogibacterium). For type B, three co-occurring bacterial genera (unclassified Clostridiaceae, Peptostreptococcus, and Clostridium) were related to mixed pigment and caries. Three dominant bacterial genera (Selenomonas, Gemella, and Streptobacillus) were linked to the presence of caries.ConclusionsOur study demonstrates that plaque-associated oral microbial communities could majorly contribute to the formation of pigment and caries in primary dentition and suggests potential clinical applications of monitoring oral microbiota as an indicator for disease diagnosis and prognosis.

【 授权许可】

CC BY   
© The Author(s). 2016

【 预 览 】
附件列表
Files Size Format View
RO202311108765738ZK.pdf 1290KB PDF download
【 参考文献 】
  • [1]
  • [2]
  • [3]
  • [4]
  • [5]
  • [6]
  • [7]
  • [8]
  • [9]
  • [10]
  • [11]
  • [12]
  • [13]
  • [14]
  • [15]
  • [16]
  • [17]
  • [18]
  • [19]
  • [20]
  • [21]
  • [22]
  • [23]
  • [24]
  • [25]
  • [26]
  • [27]
  • [28]
  • [29]
  • [30]
  • [31]
  • [32]
  • [33]
  • [34]
  • [35]
  • [36]
  • [37]
  • [38]
  • [39]
  • [40]
  • [41]
  • [42]
  • [43]
  • [44]
  • [45]
  • [46]
  • [47]
  • [48]
  • [49]
  • [50]
  文献评价指标  
  下载次数:1次 浏览次数:1次