Down-regulation of HSP70 sensitizes gastric epithelial cells to apoptosis and growth retardation triggered by H. pylori

BMC Gastroenterology

Research Article

Yan Chen¹  Gaofeng Lu²  Weili Liu³  Jianmin Si³  Leimin Sun³ 

[1] Department of Gastroenterology, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China;Department of thoracic surgery, Zhejiang Hospital, Hangzhou, China;Gastroenterology laboratory, Clinical Research Institute, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, 310016, Hangzhou, China;

关键词: Pylorus Infection;    Atrophic Gastritis;    HSP70 Expression;    Gastric Epithelial Cell;    Gastric Cell;   

DOI  :  10.1186/1471-230X-11-146

 received in 2011-08-29, accepted in 2011-12-30,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundH. pylori infection significantly attenuated the expression of HSP70 in gastric mucosal cells. However, the role of HSP70 cancellation in H. pylori-associated cell damages is largely unclear.MethodsSmall interfering RNA (siRNA) was used to down-regulate HSP70 in gastric epithelial cell lines AGS. The transfected cells were then incubated with H. pylori and the functions of HSP70 suppression were observed by viability assay, cell cycle analyses and TUNEL assay. HSP70 target apoptotic proteins were further identified by Western blot.ResultsThe inhibition of HSP70 has further increased the effect of growth arrest and apoptosis activation triggered by H. pylori in gastric epithelial cells. The anti-proliferation function of HSP70 depletion was at least by up-regulating p21 and cell cycle modulation with S-phase accumulation. An increase of apoptosis-inducing factor (AIF) and cytosolic cytochrome C contributes to the activation of apoptosis following down-regulation of intracellular HSP70. Extracellular HSP70 increased cellular resistance to apoptosis by suppression the release of AIF and cytochrome c from mitochondria, as well as inhibition of p21 expression.ConclusionsThe inhibition of HSP70 aggravated gastric cellular damages induced by H. pylori. Induction of HSP70 could be a potential therapeutic target for protection gastric mucosa from H. pylori-associated injury.

【 授权许可】

Unknown   
© Liu et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.