Transcriptome of the adult female malaria mosquito vector Anopheles albimanus

BMC Genomics

Research Article

Lilia González-Cerón¹  Salvador Hernández-Martínez²  Juan Téllez-Sosa²  Jesús Martínez-Barnetche²  Marbella Ovilla-Muñoz²  David E García López²  Mario H Rodríguez López²  Rosa E Gómez-Barreto²  Febe E Cazares-Raga³  Antonis Rokas⁴  John G Gibbons⁴  Carlos A Machado⁵  Robert M MacCallum⁶  Rhoel R Dinglasan⁷  Ceereena Ubaida Mohien⁸  Seth N Redmond⁹ 

[1] Centro Regional de Investigación en Salud Pública, Instituto Nacional de Salud Pública, Tapachula, Chiapas, México;Centro de Investigación sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, México;Departamento de Infectómica y Patogénesis Molecular, Cinvestav-IPN, México, DF, México;Department of Biological Sciences, Vanderbilt University, Nashville, TN, USA;Department of Biology, University of Maryland, College Park, MD, USA;Division of Cell and Molecular Biology, Department of Life Sciences, Imperial College London, London, United Kingdom;Johns Hopkins Bloomberg School of Public Health. Department of Molecular Microbiology & Immunology, Johns Hopkins Malaria Research Institute, 21205, Baltimore, MD, USA;Johns Hopkins Bloomberg School of Public Health. Department of Molecular Microbiology & Immunology, Johns Hopkins Malaria Research Institute, 21205, Baltimore, MD, USA;Department of Molecular & Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, MD, USA;Pasteur Institut, 28 Rue Du Docteur Roux, 75015, Paris, France;

关键词: Anopheles albimanus;    Transcriptome;    Malaria;    RNA-Seq;   

DOI  :  10.1186/1471-2164-13-207

 received in 2012-02-15, accepted in 2012-05-30,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundHuman Malaria is transmitted by mosquitoes of the genus Anopheles. Transmission is a complex phenomenon involving biological and environmental factors of humans, parasites and mosquitoes. Among more than 500 anopheline species, only a few species from different branches of the mosquito evolutionary tree transmit malaria, suggesting that their vectorial capacity has evolved independently. Anopheles albimanus (subgenus Nyssorhynchus) is an important malaria vector in the Americas. The divergence time between Anopheles gambiae, the main malaria vector in Africa, and the Neotropical vectors has been estimated to be 100 My. To better understand the biological basis of malaria transmission and to develop novel and effective means of vector control, there is a need to explore the mosquito biology beyond the An. gambiae complex.ResultsWe sequenced the transcriptome of the An. albimanus adult female. By combining Sanger, 454 and Illumina sequences from cDNA libraries derived from the midgut, cuticular fat body, dorsal vessel, salivary gland and whole body, we generated a single, high-quality assembly containing 16,669 transcripts, 92% of which mapped to the An. darlingi genome and covered 90% of the core eukaryotic genome. Bidirectional comparisons between the An. gambiae, An. darlingi and An. albimanus predicted proteomes allowed the identification of 3,772 putative orthologs. More than half of the transcripts had a match to proteins in other insect vectors and had an InterPro annotation. We identified several protein families that may be relevant to the study of Plasmodium-mosquito interaction. An open source transcript annotation browser called GDAV (Genome-Delinked Annotation Viewer) was developed to facilitate public access to the data generated by this and future transcriptome projects.ConclusionsWe have explored the adult female transcriptome of one important New World malaria vector, An. albimanus. We identified protein-coding transcripts involved in biological processes that may be relevant to the Plasmodium lifecycle and can serve as the starting point for searching targets for novel control strategies. Our data increase the available genomic information regarding An. albimanus several hundred-fold, and will facilitate molecular research in medical entomology, evolutionary biology, genomics and proteomics of anopheline mosquito vectors. The data reported in this manuscript is accessible to the community via the VectorBase website (http://www.vectorbase.org/Other/AdditionalOrganisms/).

【 授权许可】

Unknown   
© Martinez-Barnetche et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.