Up-regulated expression of substance P in CD8+ T cells and NK1R on monocytes of atopic dermatitis

Journal of Translational Medicine

Research

Junling Wang¹  Shaoheng He¹  Zenan Zhang¹  Huiyun Zhang¹  Wenjiao Zheng²  Hua Xie³  Ruonan Chai³ 

[1] Allergy and Clinical Immunology Research Centre, The First Affiliated Hospital of Jinzhou Medical University, No. 2, Section 5, Renmin Street, Guta District, 121001, Jinzhou, Liaoning, People’s Republic of China;Department of Laboratory Medicine, Zibo Mining Group Co., LTD. Central Hospital, 255120, Zibo, Shandong, China;The PLA Center of Respiratory and Allergic Disease Diagnosing Management, General Hospital of Shenyang Military Area Command, 110840, Shenyang, Liaoning, China;

关键词: Allergen;    NK1R;    T cell;    Substance P;   

DOI  :  10.1186/s12967-017-1196-6

 received in 2017-01-14, accepted in 2017-04-25,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundLarge numbers of CD8+ T cells were observed in atopic dermatitis (AD) skin, and monocytes from AD patients showed increased prostaglandin E2 production. However, little is known about the expression of substance P (SP) and its receptor NK1R in blood leukocytes of patients with AD.ObjectiveTo explore the expression of SP and NK1R in leukocytes of AD and the influence of allergens on SP and NK1R expression.MethodsThe expression levels of SP and NK1R in patients with AD were examined by flow cytometry, ELISA and a mouse AD model.ResultsThe plasma SP level was 4.9-fold higher in patients with AD than in HC subjects. Both the percentage of SP expression in the population and mean fluorescence intensity (MFI) of SP expression were elevated in CD8+ T cells in the blood of AD patients. However, both the CD14+NK1R+ population and MFI of NK1R expression on CD14+ cells were enhanced in the blood of AD patients. Allergens ASWE, HDME and PPE failed to up-regulate SP expression in CD8+ T cells. However, allergens ASWE and HDME both enhanced NK1R expression on CD14+ blood leukocytes regardless of AD or HC subjects. OVA-sensitized AD mice showed an elevated proportion and MFI of SP-expressing CD8+ T cells in the blood, which agrees with the SP expression situation in human AD blood. Injection of SP into mouse skin did not up-regulate NK1R expression on monocytes.ConclusionsAn elevated plasma SP level, up-regulated expression of SP and NK1R indicate that the SP/NK1R complex is important in the development of AD. Therefore, SP and NK1R antagonist or blocker agents may help to treat patients with AD.Trial registration Registration number: ChiCTR-BOC-16010279; Registration date: Dec., 28, 2016; retrospectively registered

【 授权许可】

CC BY   
© The Author(s) 2017