Therapeutic angiogenesis by transplantation of induced pluripotent stem cell-derived Flk-1 positive cells

BMC Cell Biology

Research Article

Masakazu Ishii¹  Ping Li¹  Rei Shibata¹  Yasushi Numaguchi¹  Toyoaki Murohara¹  Hirohiko Suzuki²  Tetsutaro Kito²  Kenichi Isobe³  Naomi Nishio³  Sachiko Ito³  Toru Yoshikai³  Jun K Yamashita⁴ 

[1] Department of Cardiology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, 466-8560, Nagoya, Aichi, Japan;Department of Cardiology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, 466-8560, Nagoya, Aichi, Japan;Department of Immunology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, 466-8560, Nagoya, Aichi, Japan;Department of Immunology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, 466-8560, Nagoya, Aichi, Japan;Laboratory of Stem Cell Differentiation, Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, 53 Shogoin Kawahara-cho, Sakyo-ku, 606-8507, Kyoto, Japan;

关键词: Somatic Stem Cell;    Therapeutic Angiogenesis;    VEGF mRNA;    Hind Limb Ischemia;    Severe Combine Immune Deficiency Mouse;   

DOI  :  10.1186/1471-2121-11-72

 received in 2010-01-04, accepted in 2010-09-22,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundInduced pluripotent stem (iPS) cells are the novel stem cell population induced from somatic cells. It is anticipated that iPS will be used in the expanding field of regenerative medicine. Here, we investigated whether implantation of fetal liver kinase-1 positive (Flk-1+) cells derived from iPS cells could improve angiogenesis in a mouse hind limb model of ischemia.ResultsFlk-1+ cells were induced from iPS cells after four to five days of culture. Hind limb ischemia was surgically induced and sorted Flk-1+ cells were directly injected into ischemic hind limbs of athymic nude mice. Revascularization of the ischemic hind limb was accelerated in mice that were transplanted with Flk-1+ cells compared with control mice, which were transplanted with vehicle, as evaluated by laser Doppler blood flowmetry. Transplantation of Flk-1+ cells also increased expression of VEGF mRNA in ischemic tissue compared to controls.ConclusionsDirect local implantation of iPS cell-derived Flk-1+ cells would salvage tissues from ischemia. These data indicate that iPS cells could be valuable in the therapeutic induction of angiogenesis.

【 授权许可】

Unknown   
© Suzuki et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.