Activation of the transcription factor, nuclear factor kappa-B, during the estrous cycle and early pregnancy in the pig

Reproductive Biology and Endocrinology

Research

Patrick Reagan¹  Morgan D Ashworth¹  Jason W Ross²  Rodney D Geisert³  Thomas E Spencer⁴  Kanako Hayashi⁵  Matthew Lucy⁶  Daniel Mathew⁶  Jerry W Ritchey⁷ 

[1] Department of Animal Science, Oklahoma State University, 74078, Stillwater, OK, USA;Department of Animal Science, Oklahoma State University, 74078, Stillwater, OK, USA;Iowa State University, College of Agriculture and Life Sciences, Department of Animal Science, 50011, Ames, IA, USA;Department of Animal Science, Oklahoma State University, 74078, Stillwater, OK, USA;University of Missouri-Columbia, College of Agriculture, Food and Natural Resources, Animal Science Division, 65203, Columbia, MO, USA;Department of Animal Science, Texas A&M University, College Station, 77843, TX, USA;Department of Animal Science, Texas A&M University, College Station, 77843, TX, USA;Department of Physiology, Southern Illinois University School of Medicine, 62901, Carbondale, IL, USA;Department of Animal Science, University of Missouri, 65211, Columbia, MO, USA;Department of Veterinary Pathobiology, Oklahoma State University, 74078, Stillwater, Oklahoma, USA;

关键词: Estrous Cycle;    Leukaemia Inhibitory Factor;    NFKB Activation;    Luminal Epithelium;    Uterine Receptivity;   

DOI  :  10.1186/1477-7827-8-39

 received in 2009-11-07, accepted in 2010-04-28,  发布年份 2010

来源: Springer

【 摘 要 】

Establishment and maintenance of pregnancy in the pig involves intricate communication between the developing conceptuses and the maternal endometrium. This process occurs during trophoblast elongation which is spaciotemporally associated with conceptus synthesis and release of IL1B concomitant with pregnancy-specific endometrial up-regulation of IL-1 receptors, providing the potential for activation of the transcription factor, NFKB. The objective of the current investigation was to determine changes in expression and cellular localization of NFKB and associated factors during the estrous cycle and early pregnancy in the pig. In situ hybridization was used to localize changes in PGR, ESR1, and TNFRSF11A during the peri-implantation period. Quantitative RT-PCR was utilized to demonstrate gene expression changes for NFKB1, RELA, TNFRSF11A, TLR4, NFKBIA and NFKBIB. Transcription factor ELISA demonstrated an overall increase in RELA during the peri-implantation period in both cyclic and pregnant gilts. While the presence of TNFSF11A and TLR4 were both detected, TLR4 expression changes were temporally associated with NFKB expression and activation. Collectively, these data demonstrate that NFKB activation may occur during the period of uterine receptivity in both the cyclic and pregnant endometrium.

【 授权许可】

Unknown   
© Ross et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.