Molecular characterization and functional analysis of barley semi-dwarf mutant Riso no. 9265

BMC Genomics

Research Article

Guoping Zhang¹  Qiaojun Jia²  Jinghuan Zhu³  Jianming Yang³  Yi Shang³  Wei Hua³  Junmei Wang³  Chengdao Li⁴ 

[1] Institute of Crop Science, Zhejiang University, 310058, Hangzhou, China;Institute of Crop Science, Zhejiang University, 310058, Hangzhou, China;Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, 310021, Hangzhou, China;Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, 310021, Hangzhou, China;Western Barley Genetics Alliance, Murdoch University, WA6150, Murdoch, Australia;

关键词: Barley;    Deletion;    Gene compensation;    Gibberellin;    Semi-dwarf;   

DOI  :  10.1186/s12864-015-2116-x

 received in 2015-07-22, accepted in 2015-10-20,  发布年份 2015

来源: Springer

【 摘 要 】

Backgroundsdw1/denso is one of the most important and useful semi-dwarf genes in barley breeding. At least four sdw1/denso alleles have been reported and HvGA20ox2 is considered as the candidate gene. Up to date, results of studies have not univocally proven the genetic relationship between sdw1/denso and HvGA20ox2.ResultsIn the present study, a complete deletion of Morex_contig_40861 including both HvGA20ox2 and Mloc_56463 genes was identified at the sdw1 locus from a semi-dwarf mutant Riso no. 9265. Expression of the genes encoding gibberellin biosynthesis (HvGA20ox1 and HvGA3ox2) were increased in the mutant compared to the wild type Bomi, while the expression of GA catabolic gene HvGA2ox3 was decreased. Over-expression of HvGA20ox2 could rescue the semi-dwarf phenotype and increase GAs concentration.ConclusionsWe confirmed that a GA biosynthetic enzyme HvGA20ox2, acted as GA 20-oxidase, is the functional gene for the sdw1/denso semi-dwarfism. Lose of HvGA20ox2 is partially compensated by HvGA20ox1 and further feedback is regulated by gibberellin. We also deduced that the sdw1/denso allele itself affects later heading owing to its reduced endogenous GAs concentration.

【 授权许可】

CC BY   
© Jia et al. 2015