Evaluation of fluorescence in situ hybridisation (FISH) for the detection of fungi directly from blood cultures and cerebrospinal fluid from patients with suspected invasive mycoses

Annals of Clinical Microbiology and Antimicrobials

Research

Hagen Frickmann¹  Carla Silvana Santos²  Kátia Santana Cruz²  João Ricardo Da Silva Neto²  Sven Poppert³  João Vicente Braga De Souza⁴  Daniela Koshikene⁵  Roberto Moreira Da Silva⁶ 

[1] Department of Tropical Medicine at the Bernhard Nocht Institute, German Armed Forces Hospital of Hamburg, Hamburg, Germany;Fundação de Medicina Tropical Dr. Heitor Vieira Dourado, Manaus, Brazil;Institute of Medical Microbiology, Justus-Liebig-University Giessen, Giessen, Germany;Instituto Nacional de Pesquisas da Amazônia, Manaus, Brazil;Biotecnólogo/Tecnologista Pleno III, Instituto Nacional de Pesquisas da Amazônia, Coordenação de Sociedade, Ambiente e Saúde, Laboratório de Micologia, Av. André Araújo, 2936, Aleixo, CEP 69060-001, Manaus, AM, Brazil;Instituto de Criminalística, Manaus, Brazil;Universidade do Estado do Amazonas, Manaus, Brazil;

关键词: FISH;    Invasive mycoses;    CSF;    Blood culture;    rRNA;    Hybridisation;   

DOI  :  10.1186/s12941-015-0065-5

 received in 2014-10-07, accepted in 2015-01-18,  发布年份 2015

来源: Springer

【 摘 要 】

The aim of this study was to evaluate the diagnostic performance of in-house FISH (fluorescence in situ hybridisation) procedures for the direct identification of invasive fungal infections in blood cultures and cerebrospinal fluid (CSF) samples and to compare these FISH results with those obtained using traditional microbiological techniques and PCR targeting of the ITS1 region of the rRNA gene. In total, 112 CSF samples and 30 positive blood cultures were investigated by microscopic examination, culture, PCR-RFLP and FISH. The sensitivity of FISH for fungal infections in CSF proved to be slightly better than that of conventional microscopy (India ink) under the experimental conditions, detecting 48 (instead of 46) infections in 112 samples. The discriminatory powers of traditional microbiology, PCR-RFLP and FISH for fungal bloodstream infections were equivalent, with the detection of 14 fungal infections in 30 samples. However, the mean times to diagnosis after the detection of microbial growth by automated blood culture systems were 5 hours, 20 hours and 6 days for FISH, PCR-RFLP and traditional microbiology, respectively. The results demonstrate that FISH is a valuable tool for the identification of invasive mycoses that can be implemented in the diagnostic routine of hospital laboratories.

【 授权许可】

Unknown   
© Da Silva et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.