Highly predictive SNP markers for efficient selection of the wheat leaf rust resistance gene Lr16

BMC Plant Biology

Research Article

Frank M. You¹  Colin W. Hiebert¹  James G. Menzies¹  Curt A. McCartney¹  Brent D. McCallum¹  Mulualem T. Kassa²  D. Gavin Humphreys³  Nicole Rezac Harrison⁴  Pierre R. Fobert⁵  Andrew G. Sharpe⁶  John P. Fellers⁷  Curtis J. Pozniak⁸ 

[1] Agriculture and Agri-Food Canada, Morden Research and Development Centre, 101 Route 100, R6M 1Y5, Morden, MB, Canada;Agriculture and Agri-Food Canada, Morden Research and Development Centre, 101 Route 100, R6M 1Y5, Morden, MB, Canada;National Research Council, 110 Gymnasium Place, S7N 0W9, Saskatoon, SK, Canada;Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, 1341 Baseline Road, K1A 0C5, Ottawa, ON, Canada;Department of Plant Pathology, Kansas State University, 66506, Manhattan, KS, USA;National Research Council, 110 Gymnasium Place, S7N 0W9, Saskatoon, SK, Canada;National Research Council, 110 Gymnasium Place, S7N 0W9, Saskatoon, SK, Canada;University of Saskatchewan, Global Institute for Food Security, 110 Gymnasium Place, S7N 4J8, Saskatoon, SK, Canada;USDA–ARS, Hard Winter Wheat Genetics Research Unit, 66506, Manhattan, KS, USA;University of Saskatchewan, Crop Development Centre, 51 Campus Drive, S7N 5A8, Saskatoon, SK, Canada;

关键词: Lr16;    Leaf rust;    Puccinia triticina;    Wheat;    Triticum aestivum;    Disease resistance;    Single nucleotide polymorphism (SNP);    Linkage analysis;    Resistance gene analog (RGA);   

DOI  :  10.1186/s12870-017-0993-7

 received in 2016-09-27, accepted in 2017-01-31,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundLr16 is a widely deployed leaf rust resistance gene in wheat (Triticum aestivum L.) that is highly effective against the North American Puccinia triticina population when pyramided with the gene Lr34. Lr16 is a seedling leaf rust resistance gene conditioning an incompatible interaction with a distinct necrotic ring surrounding the uredinium. Lr16 was previously mapped to the telomeric region of the short arm of wheat chromosome 2B. The goals of this study were to develop numerous single nucleotide polymorphism (SNP) markers for the Lr16 region and identify diagnostic gene-specific SNP marker assays for marker-assisted selection (MAS).ResultsForty-three SNP markers were developed and mapped on chromosome 2BS tightly linked with the resistance gene Lr16 across four mapping populations representing a total of 1528 gametes. Kompetitive Allele Specific PCR (KASP) assays were designed for all identified SNPs. Resistance gene analogs (RGAs) linked with the Lr16 locus were identified and RGA-based SNP markers were developed. The diagnostic potential of the SNPs co-segregating with Lr16 was evaluated in a diverse set of 133 cultivars and breeding lines. Six SNP markers were consistent with the Lr16 phenotype and are accurately predictive of Lr16 for all wheat lines/cultivars in the panel.ConclusionsLr16 was mapped relative to SNP markers in four populations. Six SNP markers exhibited high quality clustering in the KASP assay and are suitable for MAS of Lr16 in wheat breeding programs.

【 授权许可】

CC BY   
© The Author(s). 2017