An analysis of the changes in soluble hydrogenase and global gene expression in Cupriavidus necator (Ralstonia eutropha) H16 grown in heterotrophic diauxic batch culture

Microbial Cell Factories

Research

Helene Lebhar¹  Bat-Erdene Jugder¹  Darren Tan Tek Ping¹  Jeffrey Welch¹  Christopher P Marquis¹  Zhiliang Chen² 

[1] School of Biotechnology and Biomolecular Sciences, University of New South Wales, 2052, Sydney, Australia;School of Biotechnology and Biomolecular Sciences, University of New South Wales, 2052, Sydney, Australia;Systems Biology Initiative, University of New South Wales, 2052, Sydney, Australia;

关键词: Soluble hydrogenase;    Ralstonia eutropha;    Cupriavidus necator;    Heterotrophic batch culture;    Controlled bioreactor;    Transcriptome;   

DOI  :  10.1186/s12934-015-0226-4

 received in 2015-01-07, accepted in 2015-03-12,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundSoluble hydrogenases (SH) are enzymes that catalyse the oxidation of molecular hydrogen. The SH enzyme from Cupriavidus necator H16 is relatively oxygen tolerant and makes an attractive target for potential application in biochemical hydrogen fuel cells. Expression of the enzyme can be mediated by derepression of the hox promoter system under heterotrophic conditions. However, the overall impact of hox derepression, from a transcriptomic perspective, has never been previously reported.ResultsDerepression of hydrogenase gene expression upon fructose depletion was confirmed in replicate experiments. Using qRT-PCR, hoxF was 4.6-fold up-regulated, hypF2 was up-regulated in the cells grown 2.2-fold and the regulatory gene hoxA was up-regulated by a mean factor of 4.5. A full transcriptomic evaluation revealed a substantial shift in the global pattern of gene expression. In addition to up-regulation of genes associated with hydrogenase expression, significant changes were observed in genes associated with energy transduction, amino acid metabolism, transcription and translation (and regulation thereof), genes associated with cell stress, lipid and cell wall biogenesis and other functions, including cell motility.ConclusionsWe report the first full transcriptome analysis of C. necator H16 grown heterotrophically on fructose and glycerol in diauxic batch culture, which permits expression of soluble hydrogenase under heterotrophic conditions. The data presented deepens our understanding of the changes in global gene expression patterns that occur during the switch to growth on glycerol and suggests that energy deficit is a key driver for induction of hydrogenase expression in this organism.

【 授权许可】

Unknown   
© Jugder et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.