Regulatory polymorphisms in the bovine Ankyrin 1 gene promoter are associated with tenderness and intramuscular fat content

BMC Genetics

Research Article

Torres Sweeney¹  Ruth M Hamill²  Anne Maria Mullen²  Ozlem Aslan³ 

[1] School of Agriculture, Food Science & Veterinary Medicine, University College Dublin (UCD), Dublin 4, Ireland;Teagasc, Food Research Centre, 15, Ashtown, Dublin, Ireland;Teagasc, Food Research Centre, 15, Ashtown, Dublin, Ireland;School of Agriculture, Food Science & Veterinary Medicine, University College Dublin (UCD), Dublin 4, Ireland;

关键词: Transcription Start Site;    Transcription Factor Binding Site;    Meat Quality;    Meat Quality Trait;    Promoter SNPs;   

DOI  :  10.1186/1471-2156-11-111

 received in 2010-08-19, accepted in 2010-12-15,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundRecent QTL and gene expression studies have highlighted ankyrins as positional and functional candidate genes for meat quality. Our objective was to characterise the promoter region of the bovine ankyrin 1 gene and to test polymorphisms for association with sensory and technological meat quality measures.ResultsSeven novel promoter SNPs were identified in a 1.11 kb region of the ankyrin 1 promoter in Angus, Charolais and Limousin bulls (n = 15 per breed) as well as 141 crossbred beef animals for which meat quality data was available. Eighteen haplotypes were inferred with significant breed variation in haplotype frequencies. The five most frequent SNPs and the four most frequent haplotypes were subsequently tested for association with sensory and technological measures of meat quality in the crossbred population. SNP1, SNP3 and SNP4 (which were subsequently designated regulatory SNPs) and SNP5 were associated with traits that contribute to sensorial and technological measurements of tenderness and texture; Haplotype 1 and haplotype 4 were oppositely correlated with traits contributing to tenderness (P < 0.05). While no single SNP was associated with intramuscular fat (IMF), a clear association with increased IMF and juiciness was observed for haplotype 2.ConclusionThe conclusion from this study is that alleles defining haplotypes 2 and 4 could usefully contribute to marker SNP panels used to select individuals with improved IMF/juiciness or tenderness in a genome-assisted selection framework.

【 授权许可】

Unknown   
© Aslan et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.