Genetic diversity, molecular phylogeny and selection evidence of the silkworm mitochondria implicated by complete resequencing of 41 genomes

BMC Evolutionary Biology

Research Article

Yiran Guo¹  Haojing Shao¹  Laurent C Tellier²  Jun Wang²  Zhonghuai Xiang³  Dong Li⁴  Qingyou Xia⁵ 

[1] BGI-Shenzhen, 518083, Shenzhen, China;BGI-Shenzhen, 518083, Shenzhen, China;Department of Biology, University of Copenhagen, Universitetsparken 15, 2100, Kbh Ø, Denmark;The Key Sericultural Laboratory of Agricultural Ministry, College of Biotechnology, Southwest University, 400715, Chongqing, China;The Key Sericultural Laboratory of Agricultural Ministry, College of Biotechnology, Southwest University, 400715, Chongqing, China;BGI-Shenzhen, 518083, Shenzhen, China;The Key Sericultural Laboratory of Agricultural Ministry, College of Biotechnology, Southwest University, 400715, Chongqing, China;Institute of Agronomy and Life Sciences, Chongqing University, 400030, Chongqing, China;

关键词: Effective Population Size;    Nuclear Genome;    Cytb Gene;    Domestication Event;    Haploid Chromosome Number;   

DOI  :  10.1186/1471-2148-10-81

 received in 2009-11-16, accepted in 2010-03-24,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundMitochondria are a valuable resource for studying the evolutionary process and deducing phylogeny. A few mitochondria genomes have been sequenced, but a comprehensive picture of the domestication event for silkworm mitochondria remains to be established. In this study, we integrate the extant data, and perform a whole genome resequencing of Japanese wild silkworm to obtain breakthrough results in silkworm mitochondrial (mt) population, and finally use these to deduce a more comprehensive phylogeny of the Bombycidae.ResultsWe identified 347 single nucleotide polymorphisms (SNPs) in the mt genome, but found no past recombination event to have occurred in the silkworm progenitor. A phylogeny inferred from these whole genome SNPs resulted in a well-classified tree, confirming that the domesticated silkworm, Bombyx mori, most recently diverged from the Chinese wild silkworm, rather than from the Japanese wild silkworm. We showed that the population sizes of the domesticated and Chinese wild silkworms both experience neither expansion nor contraction. We also discovered that one mt gene, named cytochrome b, shows a strong signal of positive selection in the domesticated clade. This gene is related to energy metabolism, and may have played an important role during silkworm domestication.ConclusionsWe present a comparative analysis on 41 mt genomes of B. mori and B. mandarina from China and Japan. With these, we obtain a much clearer picture of the evolution history of the silkworm. The data and analyses presented here aid our understanding of the silkworm in general, and provide a crucial insight into silkworm phylogeny.

【 授权许可】

Unknown   
© Li et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.