Construction of a gene knockout CHO cell line using a simple gene targeting method

BMC Proceedings

Poster Presentation

Toshitaka Kumamoto¹  Marina Aga¹  Jana Frank²  Noriko Yamano²  Masayoshi Onitsuka²  Takeshi Omasa³ 

[1] Graduate School of Advanced Technology and Science, Tokushima University, 770-8506, Tokushima, Japan;Institute of Technology and Science, Tokushima University, 770-8506, Tokushima, Japan;Institute of Technology and Science, Tokushima University, 770-8506, Tokushima, Japan;Graduate School of Engineering, Osaka University, 565-0871, Osaka, Japan;

关键词: Chinese Hamster Ovary Cell;    Exogenous Gene;    Cluster Regularly Interspaced Short Palindromic Repeat;    Chinese Hamster Ovary Cell Line;    Random Integration;   

DOI  :  10.1186/1753-6561-9-S9-P2

来源: Springer

【 授权许可】

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© Aga et al. 2015. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.