Aneuploidy analysis in day 7 human blastocysts produced by in vitro fertilization

Reproductive Biology and Endocrinology

Research

Jian-Jun Li¹  Yu Su¹  Ghassan Haddad²  Cassie Wang³  Wei-Hua Wang⁴ 

[1] Center for Reproductive Medicine, Changsha Hospital for Maternal and Child Health Care, Changsha, Hunan, China;Houston Fertility Institute, Houston, TX, USA;Vivere Health, Franklin, TN, USA;Vivere-Houston Fertility Laboratory, 2500 Fondren Road, Suite 350, Houston, TX, USA;Vivere-Houston Fertility Laboratory, 2500 Fondren Road, Suite 350, Houston, TX, USA;Vivere Health, Franklin, TN, USA;

关键词: Aneuploidy;    Delayed blastocyst;    Implantation;    PGS;   

DOI  :  10.1186/s12958-016-0157-x

 received in 2016-03-02, accepted in 2016-04-10,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundHuman embryos produced by in vitro fertilization (IVF) are usually cultured to day 6 after insemination, and good quality of embryos should develop to blastocyst stage at days 5 and 6. However, some embryos develop slowly, thus they may form blastocysts on day 7. Most IVF laboratories culture embryos to day 6 and then discard retarded embryos that do not develop to blastocyst stage. It has been reported that transfer of day 7 blastocysts can yield pregnancy although the rates were very low. In the present study, we evaluated the prevalence of aneuploidy in day 7 human blastocysts and also assessed embryo implantation after transfer of normal euploid blastocysts developed on day 7.MethodsDay 7 blastocysts were biopsied and screened for aneuploidy. Embryo implantation was assessed by transferring of euploid blastocysts.ResultsA total of 1966 blastocysts from 367 IVF cycles were biopsied and screened for aneuploidy. It was found that 81.5 % of the patients had days 5 and 6 blastocysts and 18.5 % (68) patients had blastocysts developed on day 7, including 15.3 % had days 5–7 blastocysts and 3.3 % had only day 7 blastocysts. A total of 151 day 7 blastocysts, which accounted for 7.7 % of total blastocysts, were analyzed. It was found that 36.7 % of the blastocysts were euploid and 63.3 % had abnormal chromosomes, including aneuploidy and euploid with partial chromosome deletion. The aneuploidy rate was also maternal age dependent and was as high as 91.7 % in patients who were ≥40 years old. During the study period, transfer of day 7 euploid blastocysts in 15 patients resulted in 2 healthy live births.Conclusion(s)Aneuploidy rates in day 7 human blastocysts produced by IVF are very high. However, good euploid blastocysts have potential to implant and transfer of day 7 euploid blastocysts can result in healthy live birth. It is suggested that day 7 blastocyst culture may be necessary in patients who need aneuploidy screening.

【 授权许可】

CC BY   
© Su et al. 2016