Immune signatures in human PBMCs of idiotypic vaccine for HCV-related lymphoproliferative disorders

Journal of Translational Medicine

Research

Luca Romagnoli¹  Vitor Sousa¹  Riccardo Dolcetti²  Debora Martorelli²  Gerardo Beneduce³  Valli De Re⁴  Amalia De Renzo⁵  Giuseppe Castello⁶  Maria Napolitano⁶  Franco M Buonaguro⁷  Marialina Tornesello⁷  Annacarmen Petrizzo⁷  Luigi Buonaguro⁸  Oreste Perrella⁹ 

[1] Areta International, Gerenzano, Italy;Cancer Bio-Immunotherapy Unit, Centro di Riferimento Oncologico, I.R.C.C.S. - National Cancer Institute, Aviano, Italy;Clinical Pathology, Istituto Nazionale Tumori "Fond. G. Pascale", Naples, Italy;Experimental and Clinical Pharmacology, Centro di Riferimento Oncologico, I.R.C.C.S. National Cancer Institute, Aviano, Italy;Haematology Unit, University of Naples "Federico II", School of Medicine, Naples, Italy;Lab of Clinical Immunology, Istituto Nazionale Tumori "Fond. G. Pascale", Naples, Italy;Lab. of Molecular Biology and Viral Oncogenesis & AIDS Reference Center, Istituto Nazionale Tumori "Fond. G. Pascale", Naples, Italy;Lab. of Molecular Biology and Viral Oncogenesis & AIDS Reference Center, Istituto Nazionale Tumori "Fond. G. Pascale", Naples, Italy;Institute of Human Virology, University of Maryland School of Medicine, Baltimore, MD, USA;VII Division of Infectious Diseases, Cotugno Hospital, Naples, Italy;

关键词: Mixed Cryoglobulinemia;    Multiparameter Flow Cytometry;    Multiparametric Analysis;    Mixed Cryoglobulinemia Patient;    Average Basal Level;   

DOI  :  10.1186/1479-5876-8-18

 received in 2009-12-11, accepted in 2010-02-19,  发布年份 2010

来源: Springer

【 摘 要 】

Hepatitis C virus (HCV) is one of the major risk factors for chronic hepatitis, which may progress to cirrhosis and hepatocellular carcinoma, as well as for type II mixed cryoglobulinemia (MC), which may further evolve into an overt B-cell non-Hodgkin's lymphoma (NHL).It has been previously shown that B-cell receptor (BCR) repertoire, expressed by clonal B-cells involved in type II MC as well as in HCV-associated NHL, is constrained to a limited number of variable heavy (VH)- and light (VL)-chain genes. Among these, the VK3-20 light chain idiotype has been selected as a possible target for passive as well as active immunization strategy.In the present study, we describe the results of a multiparametric analysis of the innate and early adaptive immune response after ex vivo stimulation of human immune cells with the VK3-20 protein. This objective has been pursued by implementing high-throughput technologies such as multiparameter flow cytometry and multiplex analysis of cytokines and chemokines.

【 授权许可】

CC BY   
© Buonaguro et al; licensee BioMed Central Ltd. 2010