Genome-wide association study of blast resistance in indica rice

BMC Plant Biology

Research Article

Xiaoping Yuan¹  Hanyong Yu¹  Yiping Wang¹  Yue Feng¹  Qun Xu¹  Caihong Wang¹  Xinghua Wei¹  Yaolong Yang² 

[1] State Key Laboratory of Rice Biology, China National Rice Research Institute, 310006, Hangzhou, China;State Key Laboratory of Rice Biology, China National Rice Research Institute, 310006, Hangzhou, China;College of Agricultural Sciences, Jiangxi Agricultural University, 330045, Nanchang, China;

关键词: Blast disease;    Candidate gene;    Genome-wide association study;    Oryza sativa L;    R protein;   

DOI  :  10.1186/s12870-014-0311-6

 received in 2014-07-16, accepted in 2014-10-27,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundRice blast disease is one of the most serious and recurrent problems in rice-growing regions worldwide. Most resistance genes were identified by linkage mapping using genetic populations. We extensively examined 16 rice blast strains and a further genome-wide association study based on genotyping 0.8 million single nucleotide polymorphism variants across 366 diverse indica accessions.ResultsTotally, thirty associated loci were identified. The strongest signal (Chr11_6526998, P =1.17 × 10−17) was located within the gene Os11g0225100, one of the rice Pia-blast resistance gene. Another association signal (Chr11_30606558) was detected around the QTL Pif. Our study identified the gene Os11g0704100, a disease resistance protein containing nucleotide binding site-leucine rich repeat domain, as the main candidate gene of Pif. In order to explore the potential mechanism underlying the blast resistance, we further examined a locus in chromosome 12, which was associated with CH149 (P =7.53 × 10−15). The genes, Os12g0424700 and Os12g0427000, both described as kinase-like domain containing protein, were presumed to be required for the full function of this locus. Furthermore, we found some association on chromosome 3, in which it has not been reported any loci associated with rice blast resistance. In addition, we identified novel functional candidate genes, which might participate in the resistance regulation.ConclusionsThis work provides the basis of further study of the potential function of these candidate genes. A subset of true associations would be weakly associated with outcome in any given GWAS; therefore, large-scale replication is necessary to confirm our results. Future research will focus on validating the effects of these candidate genes and their functional variants using genetic transformation and transferred DNA insertion mutant screens, to verify that these genes engender resistance to blast disease in rice.

【 授权许可】

Unknown   
© Wang et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.