The interplay between surfaces and soluble factors define the immunologic and angiogenic properties of myeloid dendritic cells

BMC Immunology

Research Article

Amritha K Venkatesh¹  Leslee Sprague¹  Ming-yu Gu¹  Harika Nandigam¹  Fabian Benencia²  Andrew Rutowski³  Maria C Courreges³  Evan Meles³  Kristen Mansfield³  Omowaleola Omosebi³  John McGinty³  Michelle Pate³  Maria Muccioli⁴ 

[1] Biomedical Engineering Program, Russ College of Engineering and Technology, Ohio University, USA;Biomedical Engineering Program, Russ College of Engineering and Technology, Ohio University, USA;Molecular and Cell Biology Program, Ohio University, USA;Department of Biomedical Sciences, College of Osteopathic Medicine, Ohio University, USA;Department of Biomedical Sciences, College of Osteopathic Medicine, Ohio University, USA;Molecular and Cell Biology Program, Ohio University, USA;

关键词: Vascular Endothelial Growth Factor;    Hepatocyte Growth Factor;    Vascular Endothelial Growth Factor Level;    Costimulatory Molecule;    Bone Marrow Precursor;   

DOI  :  10.1186/1471-2172-12-35

 received in 2010-10-23, accepted in 2011-06-06,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundDendritic cells (DCs) are antigen presenting cells capable of inducing specific immune responses against microbial infections, transplant antigens, or tumors. Interestingly, microenvironment conditions such as those present in tumor settings might induce a DC phenotype that is poorly immunogenic and with the capability of promoting angiogenesis. We hypothesize that this plasticity may be caused not only by the action of specific cytokines or growth factors but also by the properties of the surfaces with which they interact, such as extracellular matrix (ECM) components.ResultsHerewith we studied the effect of different surfaces and soluble factors on the biology of DCs. To accomplish this, we cultured murine myeloid(m) DCs on surfaces coated with fibronectin, collagen I, gelatin, and Matrigel using poly-D-lysine and polystyrene as non-biological surfaces. Further, we cultured these cells in the presence of regular DC medium (RPMI 10% FBS) or commercially available endothelial medium (EGM-2). We determined that mDCs could be kept in culture up to 3 weeks in these conditions, but only in the presence of GM-CSF. We were able to determine that long-term DC cultures produce an array of angiogenic factors, and that some of these cultures still retain the capability to induce T cell responses.ConclusionsAltogether these data indicate that in order to design DC-based vaccines or treatments focused on changing the phenotype of DCs associated with diseases such as cancer or atherosclerosis, it becomes necessary to fully investigate the microenvironment in which these cells are present or will be delivered.

【 授权许可】

Unknown   
© Sprague et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.