Protective effects of thalidomide on pulmonary injuries in a rat model of paraquat intoxication

Journal of Inflammation

Research

Ke-Xiang Liu¹  Xiao-Wei Zhang²  Yan-Hui Li³  Xing-Hai Chen³  Xing-Quan Jiang³  Yong-Jie Yin³  Dan Li⁴  Cheng-Bo Sun⁵  Zhe Fan⁶ 

[1]Department of Cardiovascular Surgery, The Second Hospital of Jilin University, No. 218 Ziqiang Street, 130041, Changchun, Nanguan District, People’s Republic of China

[2]Department of Clinical Laboratory, Children’s Hospital of Changchun City, No. 69 Beian Road, 130061, Changchun, Chaoyang District, People’s Republic of China

[3]Department of Emergency Medicine, The Second Hospital of Jilin University, No. 218 Ziqiang Street, 130041, Changchun, Nanguan District, People’s Republic of China

[4]Department of Emergency Medicine, The Second Hospital of Jilin University, No. 218 Ziqiang Street, 130041, Changchun, Nanguan District, People’s Republic of China

[5]Department of Cardiovascular Surgery, The Second Hospital of Jilin University, No. 218 Ziqiang Street, 130041, Changchun, Nanguan District, People’s Republic of China

[6]Department of Experimental Pharmacology and Toxicology, School of Pharmacy, Jilin University, No. 1163 Xinmin Avenue, 130021, Changchun, Chaoyang District, People’s Republic of China

[7]Department of Technology, People’s Procuratorate of Jilin Province, No. 1333 Jingyang Road, 130062, Changchun, Lvyuan District, People’s Republic of China

关键词: Paraquat;    Lung injury;    Thalidomide;    Protective effects;   

DOI  :  10.1186/s12950-015-0093-0

 received in 2015-01-26, accepted in 2015-07-23,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThis study was designed to evaluate the protective effects of thalidomide on paraquat (PQ)-induced lung injuries in a rat model and to explore the underlying mechanisms.MethodsRats were exposed to 50 mg/kg PQ by oral gavage, and treated with thalidomide through oral administration at 60 mg/kg once a day, 6 days/week for 2 weeks. Serum levels of IL-6, TNF-alpha, TGFbeta1 and COL1A1 were detected at different time points after paraquat exposure. At the end of the study, lung tissues were collected for pathological inspection as well as analyses of water content and expression levels of IL-6, TNF-alpha, TGFbeta1 and COL1A1 mRNA.ResultsThe results showed that thalidomide treatment could significantly alleviate PQ-induced pathological changes in lung tissue and severity of lung edema. Thalidomide treatment after PQ exposure resulted in significantly reduced serum levels of IL-6, TNF-alpha, TGF-beta1 and COL1A1, as compared to PQ group. PCR analysis demonstrated that expression levels of IL-6, TNF-alpha, TGF-beta1 and COL1A1 in lung tissue were significantly increased after PQ exposure but reduced by thalidomide, which were confirmed by immunohistochemistry staining.ConclusionsOur results indicated that inflammatory factors played important roles in PQ-induced lung injuries and thalidomide could protect rats from PQ-induced lung injuries by inhibiting the upregulation of inflammatory factors.

【 授权许可】

Unknown   
© Li et al. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.