Identification of differentially expressed microRNAs in human male breast cancer

BMC Cancer

Research Article

Thomas Streichert¹  Benjamin Otto¹  Ursula Hille²  Elisa Schipper³  Hans H Kreipe³  Ulrich Lehmann³  Britta Hasemeier³  Florian Länger³  Cord Albat³  Henriette Christgen³ 

[1] Department of Clinical Chemistry/Central Laboratories, Universitätsklinikum Hamburg-Eppendorf, D-20246, Hamburg, Germany;Department of Gynecology, Medizinische Hochschule Hannover, D-30625, Hannover, Germany;Institute of Pathology, Medizinische Hochschule Hannover, D-30625, Hannover, Germany;

关键词: Breast Cancer;    microRNA Expression;    Male Breast Cancer;    Female Breast Cancer;    Training Cohort;   

DOI  :  10.1186/1471-2407-10-109

 received in 2009-09-11, accepted in 2010-03-23,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundThe discovery of small non-coding RNAs and the subsequent analysis of microRNA expression patterns in human cancer specimens have provided completely new insights into cancer biology. Genetic and epigenetic data indicate oncogenic or tumor suppressor function of these pleiotropic regulators. Therefore, many studies analyzed the expression and function of microRNA in human breast cancer, the most frequent malignancy in females. However, nothing is known so far about microRNA expression in male breast cancer, accounting for approximately 1% of all breast cancer cases.MethodsThe expression of 319 microRNAs was analyzed in 9 primary human male breast tumors and in epithelial cells from 15 male gynecomastia specimens using fluorescence-labeled bead technology. For identification of differentially expressed microRNAs data were analyzed by cluster analysis and selected statistical methods.Expression levels were validated for the most up- or down-regulated microRNAs in this training cohort using real-time PCR methodology as well as in an independent test cohort comprising 12 cases of human male breast cancer.ResultsUnsupervised cluster analysis separated very well male breast cancer samples and control specimens according to their microRNA expression pattern indicating cancer-specific alterations of microRNA expression in human male breast cancer. miR-21, miR519d, miR-183, miR-197, and miR-493-5p were identified as most prominently up-regulated, miR-145 and miR-497 as most prominently down-regulated in male breast cancer.ConclusionsMale breast cancer displays several differentially expressed microRNAs. Not all of them are shared with breast cancer biopsies from female patients indicating male breast cancer specific alterations of microRNA expression.

【 授权许可】

CC BY   
© Lehmann et al; licensee BioMed Central Ltd. 2010