Chlorfenapyr: a new insecticide with novel mode of action can control pyrethroid resistant malaria vectors

Malaria Journal

Research

Poonam Sharma¹  Uragayala Sreehari¹  Kamaraju Raghavendra¹  Tapan K Barik¹  Aditya P Dash²  Harish C Srivastava³  Rajendra M Bhatt⁴ 

[1] National Institute of Malaria Research (ICMR), Sector 8, 110 077, Dwarka, New Delhi, India;National Institute of Malaria Research (ICMR), Sector 8, 110 077, Dwarka, New Delhi, India;World Health Organization, South East Asia Regional Office, Indraprastha Estate, 110002, New Delhi, India;National Institute of Malaria Research Field Unit, Nadiad, Gujarat, India;National Institute of Malaria Research Field Unit, Raipur, Chhattisgarh, India;

关键词: Malathion;    Malaria Vector;    Malaria Vector Control;    Piperonyl Butoxide;    Diagnostic Dose;   

DOI  :  10.1186/1475-2875-10-16

 received in 2010-10-06, accepted in 2011-01-25,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundMalaria vectors have acquired widespread resistance to many of the currently used insecticides, including synthetic pyrethroids. Hence, there is an urgent need to develop alternative insecticides for effective management of insecticide resistance in malaria vectors. In the present study, chlorfenapyr was evaluated against Anopheles culicifacies and Anopheles stephensi for its possible use in vector control.MethodsEfficacy of chlorfenapyr against An. culicifacies and An. stephensi was assessed using adult bioassay tests. In the laboratory, determination of diagnostic dose, assessment of residual activity on different substrates, cross-resistance pattern with different insecticides and potentiation studies using piperonyl butoxide were undertaken by following standard procedures. Potential cross-resistance patterns were assessed on field populations of An. culicifacies.ResultsA dose of 5.0% chlorfenapyr was determined as the diagnostic concentration for assessing susceptibility applying the WHO tube test method in anopheline mosquitoes with 2 h exposure and 48 h holding period. The DDT-resistant/malathion-deltamethrin-susceptible strain of An. culicifacies species C showed higher LD50 and LD99 (0.67 and 2.39% respectively) values than the DDT-malathion-deltamethrin susceptible An. culicifacies species A (0.41 and 2.0% respectively) and An. stephensi strains (0.43 and 2.13% respectively) and there was no statistically significant difference in mortalities among the three mosquito species tested (p > 0.05). Residual activity of chlorfenapyr a.i. of 400 mg/m2 on five fabricated substrates, namely wood, mud, mud+lime, cement and cement + distemper was found to be effective up to 24 weeks against An. culicifacies and up to 34 weeks against An. stephensi. No cross-resistance to DDT, malathion, bendiocarb and deltamethrin was observed with chlorfenapyr in laboratory-reared strains of An. stephensi and field-caught An. culicifacies. Potentiation studies demonstrated the antagonistic effect of PBO.ConclusionLaboratory studies with susceptible and resistant strains of An. culicifacies and An. stephensi, coupled with limited field studies with multiple insecticide-resistant An. culicifacies have shown that chlorfenapyr can be a suitable insecticide for malaria vector control, in multiple-insecticide-resistant mosquitoes especially in areas with pyrethroid resistant mosquitoes.

【 授权许可】

CC BY   
© Raghavendra et al; licensee BioMed Central Ltd. 2011