Enteropathogens in children less than 5 years of age with acute diarrhea: a 5-year surveillance study in the Southeast Coast of China

BMC Infectious Diseases

Research Article

Yongzhang Cheng¹  Dongsheng Han²  Dawei Cui³  Fei Yu³  Xianzhi Yang³  Guoliang Xie³  Yiyin Wang³  Wen Zhang³  Xiao Chen⁴  Shufa Zheng⁴  Yu Chen⁴ 

[1] Center of Clinical Laboratory, Affiliated Children’s Hospital, School of Medicine, Zhejiang University, No. 57, Zhuganxiang Road, 310052, Hangzhou, People’s Republic China;Department of Clinical Laboratory, Northern Jiangsu People’s Hospital, No. 98, Nantong West Road, 225001, Yangzhou, People’s Republic China;Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, No. 79, Qingchun Road, 310003, Hangzhou, People’s Republic China;State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, No. 79, Qingchun Road, 310003, Hangzhou, People’s Republic China;Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, No. 79, Qingchun Road, 310003, Hangzhou, People’s Republic China;

关键词: Diarrhea;    Enteropathogens;    Children;    Drug resistance;   

DOI  :  10.1186/s12879-016-1760-3

 received in 2015-11-09, accepted in 2016-08-07,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundDiarrhea is the second most common cause of death among children less than 5 years of age worldwide. The etiological agents of diarrhea in the southeast coastal area of China were studied from July 2009 to December 2014.MethodsA total of the 2318 patients were enrolled in this study and examined for the presence of viruses, bacteria, and parasites. Multiplex real-time PCR was used for the detection of diarrheagenic Escherichia.coli (DEC). DEC strains were tested for susceptibility to a panel of 20 antibiotics using the Kirby-Bauer disc-diffusion method.ResultsOf the 2318 children with diarrhea, 962 (41.5 %) were positive for at least one pathogen. Rotavirus, human calicivirus (HucV), and DEC were predominant, with detection rates of 19.1 % (443), 17.7 % (411), and 7.6 % (177), respectively. The prevalences of various pathogens in patients of different ages and in different seasons were not the same. The resistance rates of 177 strains of DEC to ampicillin, tetracycline, and cefazolin were 93.2 %, 60.0 %, and 57.7 %, respectively.ConclusionsRotavirus, HucV, and DEC were the main pathogens associated with diarrhea in Zhejiang, China. DEC possessed high levels of antibiotic resistance. Increased monitoring of etiological agents of diarrhea is necessary.

【 授权许可】

CC BY   
© The Author(s). 2016