Application of avidin-biotin technology to improve cell adhesion on nanofibrous matrices

Journal of Nanobiotechnology

Research

Lin-yuan Shu¹  Jian-feng Pan²  Hui Sun²  Ning-hua Liu² 

[1] Department of Emergency, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, 600 YiShan Road, 200233, Shanghai, China;Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, 600 YiShan Road, 200233, Shanghai, China;

关键词: Electrospun nanofibers;    PLCL/Pluronic;    Avidin;    Biotin;   

DOI  :  10.1186/s12951-015-0096-2

 received in 2015-01-09, accepted in 2015-05-01,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundElectrospinning is an easy and effective technique to produce submicron fibers possessing a range of attractive characteristics such as interconnected porous structures similar to natural ECM and good resilience to movement. Rapid and efficient cell attachment to nanofibrous matrices is a necessary prerequisite in tissue engineering. Thus, the aim of this study is to evaluate poly(ε-caprolactone-co-lactide)/Pluronic (PLCL/Pluronic) nanofibrous matrices with avidin-biotin technology for improving cell adhesion for the first time.ResultsPLCL/Pluronic nanofibers had relatively homogeneous fibers and interconnected porous structures. Pluronic significantly modified the hydrophilicity of nanofibrous matrices and PLCL/Pluronic nanofibrous matrices had better performance on maintaining cell proliferation. Avidin-biotin technology had no negative effect on the hydrophilic property, mechanical property and cell proliferation. Meanwhile, the attachment and spreading of adipose-derived stem cells (ADSCs) onto PLCL/Pluronic nanofibrous matrices with avidin-biotin technology was promoted obviously.ConclusionsPLCL/Pluronic nanofibrous matrices inheriting the excellent characteristics of both PLCL and Pluronic have the better cell adhesion ability through avidin-biotin technology, implying a promising application in skin care, tissue regeneration and other related area.

【 授权许可】

Unknown   
© Pan et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.