High expression of carbonic anhydrase IX is significantly associated with glandular lesions in gastroesophageal junction and with tumorigenesis markers BMI1, MCM4 and MCM7

BMC Gastroenterology

Research Article

Jun Sun¹  Tongtong Wu²  Zhongren Zhou³  Aaron R. Huber³  Dongfeng Tan⁴  David Dean⁵ 

[1] Biochemistry, Rush University Medical Center, Chicago, IL, USA;Department of Biostatistics and Computational Biology, University of Rochester, Rochester, NY, USA;Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Box 626, 601 Elmwood Avenue, 14642, Rochester, NY, USA;Department of Pathology, MD Anderson Cancer Institute, Houston, TX, USA;Department of Pediatrics, University of Rochester, Rochester, NY, USA;

关键词: CA9;    Esophageal adenocarcinoma;    Esophageal squamous cell carcinoma;    Barrett’s esophagus;    Gastroesophageal reflux disease;    BMI1;   

DOI  :  10.1186/s12876-015-0310-6

 received in 2015-06-15, accepted in 2015-06-29,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundCarbonic anhydrase IX (CA9) is a transmembrane glycoprotein related to hypoxia. Increased CA9 expression has been associated with decreased survival and cancer progression and has been targeted as a potential therapy for several cancers, including esophageal cancer. The reported percentages of expression of CA9 in esophageal adenocarcinoma vary, and CA9 expression in precancerous esophageal lesions has not been well studied.MethodsIn this study, we investigated CA9 expression in esophageal cancers and in precancerous lesions and explored the association of CA9 expression with prognostic factors and with stem cell and tumorigenesis-related markers including BMI1, cyclin E, ki67, MCM4 and MCM7 expression. Previously constructed tissue microarrays consisting of samples of 7 tissue types (columnar cell metaplasia, Barrett esophagus, low- and high-grade dysplasia, esophageal adenocarcinoma, squamous epithelium, and squamous cell carcinoma) were used for the immunostaining of CA9, BMI1, cyclin E, Ki67, MCM4 and MCM7.Results and discussionCA9 high expression occurred more frequently in glandular mucosa with or without dysplasia than in squamous epithelium or squamous cell carcinoma. Survival duration of esophageal adenocarcinoma did not significantly differ between patients with high CA9 expression and those with low expression. High CA9 expression is significantly associated with BMI1, cyclin E, Ki67, MCM4 and MCM7 expression.ConclusionsHigh CA9 expression may be related to the acidic environment caused by gastroesophageal reflux disease in the gastroesophageal junction and associated with tumorigenesis through BMI1, MCM4 and MCM7.

【 授权许可】

Unknown   
© Huber et al. 2015. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.