Preliminary reference intervals and the impact of citrate storage time for thrombelastography in cats including delta and the velocity curve

BMC Veterinary Research

Research Article

Franziska Barthel¹  Andreas Moritz²  Natali Bauer²  Carolin Engelen² 

[1] Bayer Animal Health GmbH, Alfred-Nobel-Straße 50, 40789, Monheim am Rhein, Germany;Department of Veterinary Clinical Sciences, Clinical Pathophysiology and Clinical Pathology, Justus-Liebig University Giessen, Frankfurterstraße 126, 35392, Giessen, Germany;

关键词: Feline;    Hypercoagulable;    TEG;    Velocity curve;   

DOI  :  10.1186/s12917-017-1278-y

 received in 2016-12-22, accepted in 2017-11-16,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThrombelastography is a useful tool in assessment of hemostasis. Beside the traditional variables, the velocity curve and the variable delta have lately earned attention. The velocity curve provides knowledge about the speed of clot formation including information about thrombin generation. Delta, which only reflects enzymatic coagulation, allows the determination of the origin of hypercoagulability when compared to clot rigidity, a variable that reflects both platelet and enzymatic activity. The aim was to establish preliminary reference intervals for feline thrombelastography including the velocity curve variables and delta obtained after 60 min of storage including the assessment of coefficients of variation. Furthermore, the effect of citrate storage time (30 versus 60 min) on feline thrombelastography will be determined.ResultsProlonged storage times significantly reduced reaction (R) (P = 0.019) and clotting (K) (P = 0.008) times, split point (SP) (P = 0.019) and time to maximum rate of thrombus generation (TMRTG) (P = 0.023) values whereas maximum rate of thrombus generation (MRTG) significantly increased (P = 0.040). Preliminary reference intervals: R (min): 2.7–18.1; K (min): 0.8–3.9; alpha (°): 27.6–75.2; maximum amplitude (mm): 18.5–62.5; clot rigidity (dyn/cm2): 1.2–8.2; coagulation index: −4.6 – 2.6; SP (min): 2.4–15.4; delta (min): 0.3–3.1; thrombus generation (mm/min): 255.3–751.2; MRTG (mm/min): 4.0–19.3; TMRTG (min): 3.5–22.0; maximum rate of lysis (mm/min): 0.0–4.7 and time to maximum rate of lysis (min): 0.4–55.8.ConclusionStorage for 60 versus 30 min induces hypercoagulable tracings including the velocity curve, some of which variables (MRTG, TMRTG) might function as sensitive markers for changes in the coagulation activity. Because of the impact of citrate storage time on thrombelastography, reference intervals have to be established using a specific and constant storage time in each laboratory.

【 授权许可】

CC BY   
© The Author(s). 2017