Phenotypic convergence in bacterial adaptive evolution to ethanol stress

BMC Evolutionary Biology

Research Article

Takashi Hirasawa¹  Hiroshi Shimizu²  Tetsuya Yomo³  Naoaki Ono⁴  Takaaki Horinouchi⁵  Shingo Suzuki⁵  Chikara Furusawa⁶ 

[1] Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, 226-8501, Midori-ku, Yokohama, Japan;Graduate School of Information Science and Technology, Osaka University, 1-5 Yamada-oka, 565-0871, Suita, Osaka, Japan;Graduate School of Information Science and Technology, Osaka University, 1-5 Yamada-oka, 565-0871, Suita, Osaka, Japan;Graduate School of Information Science and Technology, Osaka University, 1-5 Yamada-oka, 565-0871, Suita, Osaka, Japan;Graduate School of Frontier Biosciences, Osaka University, 1-5 Yamada-oka, 565-0871, Suita, Osaka, Japan;Graduate School of Information Science, Nara Institute of Science and Technology, 630-0192, Ikoma, Nara, Japan;Quantitative Biology Center (QBiC), RIKEN, 6-2-3 Furuedai, 565-0874, Suita, Osaka, Japan;Quantitative Biology Center (QBiC), RIKEN, 6-2-3 Furuedai, 565-0874, Suita, Osaka, Japan;Graduate School of Information Science and Technology, Osaka University, 1-5 Yamada-oka, 565-0871, Suita, Osaka, Japan;

关键词: Parent Strain;    Adaptive Evolution;    Ethanol Tolerance;    Evolutionary Constraint;    Laboratory Evolution;   

DOI  :  10.1186/s12862-015-0454-6

 received in 2015-02-23, accepted in 2015-08-12,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundBacterial cells have a remarkable ability to adapt to environmental changes, a phenomenon known as adaptive evolution. During adaptive evolution, phenotype and genotype dynamically changes; however, the relationship between these changes and associated constraints is yet to be fully elucidated.ResultsIn this study, we analyzed phenotypic and genotypic changes in Escherichia coli cells during adaptive evolution to ethanol stress. Phenotypic changes were quantified by transcriptome and metabolome analyses and were similar among independently evolved ethanol tolerant populations, which indicate the existence of evolutionary constraints in the dynamics of adaptive evolution. Furthermore, the contribution of identified mutations in one of the tolerant strains was evaluated using site-directed mutagenesis. The result demonstrated that the introduction of all identified mutations cannot fully explain the observed tolerance in the tolerant strain.ConclusionsThe results demonstrated that the convergence of adaptive phenotypic changes and diverse genotypic changes, which suggested that the phenotype–genotype mapping is complex. The integration of transcriptome and genome data provides a quantitative understanding of evolutionary constraints.

【 授权许可】

CC BY   
© Horinouchi et al. 2015