Effect of taurine chloramine on the production of matrix metalloproteinases (MMPs) in adiponectin- or IL-1β-stimulated fibroblast-like synoviocytes

Journal of Biomedical Science

Review

Chaekyun Kim¹  Da Hee Oh²  Kyoung Soo Kim²  Hyun-Mi Choi²  Myung Chul Yoo³  Hyung-In Yang⁴  Jin Seok Jeong⁵ 

[1] Center for Advanced Medical Education by BK21 Project, Inha University School of Medicine, Incheon, Republic of Korea;East-West Bone & Joint Research Center, East-West Neo Medical Center, Kyung Hee University, 149 Sangil-dong, 137-727, Gangdong-gu, Seoul, Republic of Korea;East-West Bone & Joint Research Center, East-West Neo Medical Center, Kyung Hee University, 149 Sangil-dong, 137-727, Gangdong-gu, Seoul, Republic of Korea;Department of Orthopedic Surgery, East-West Neo Medical Center, Kyung Hee University, 149 Sangil-dong, 137-727, Gangdong-gu, Seoul, Republic of Korea;East-West Bone & Joint Research Center, East-West Neo Medical Center, Kyung Hee University, 149 Sangil-dong, 137-727, Gangdong-gu, Seoul, Republic of Korea;Divison of Rheumatology, Department of Internal Medicine, East-West Neo Medical Center, Kyung Hee University, 149 Sangil-dong, 137-727, Gangdong-gu, Seoul, Republic of Korea;Research Institute, Dong-A Pharm. Co., Ltd., 47-5 Sangal-dong, 446-905, Yongin-si, Kyunggi-do, Republic of Korea;

关键词: Rheumatoid Arthritis;    Rheumatoid Arthritis Patient;    Taurine;    Arthritic Joint;    Rheumatoid Arthritis FLSs;   

DOI  :  10.1186/1423-0127-17-S1-S27

来源: Springer

【 摘 要 】

BackgroundAdiponectin greatly stimulated the expression of matrix metalloproteinases (MMPs) in fibroblast-like synoviocytes (FLSs) as did IL-1β. We wondered whether taurine chloramine (TauCl) inhibits the production of MMPs stimulated by adiponectin in the same pattern as by IL-1β stimulation in vitroMethodsSynovial cells from rheumatoid arthritis (RA) patients were treated with adiponectin or interleukin (IL)-1β for 24 hr in the presence or absence of TauCl. The culture supernatant was collected and the levels of MMPs were measured by enzyme-linked immunosorbent assay (ELISA). The IκB signaling pathways stimulated by adiponectin were studied and the levels of NF-κB in the nuclei of the cells were analyzed by ELISA.ResultsTauCl (600 µM) inhibited MMP-13, but not MMP-1, expression in IL-1β-stimulated RA FLSs. However, TauCl at the same concentration significantly inhibited the production of both adiponectin-stimulated MMP-1 and MMP-13 expression. TauCl inhibited the degradation of IκB-α stimulated by adiponectin, but not by IL-1β. Similarly, the level of NF-κB in the nucleus was increased by adiponectin stimulation and was inhibited by 600 µM TauCl. However, the levels of NF-κB increased by IL-1β stimulation were not inhibited by 600 µM TauCl.ConclusionsTauCl more effectively inhibited MMPs expression induced by adiponectin than that by IL-1β in RA FLS, suggesting that TauCl plays an important role in down-regulating the expression of MMPs in arthritic joints.

【 授权许可】

CC BY   
© Kim et al; licensee BioMed Central Ltd. 2010