Global gene expression reveals stress-responsive genes in Aspergillus fumigatus mycelia

BMC Genomics

Research Article

Kanae Sakai¹  Yoko Kusuya¹  Daisuke Hagiwara¹  Azusa Takahashi-Nakaguchi¹  Tohru Gonoi¹  Hiroki Takahashi² 

[1] Medical Mycology Research Center, Chiba University, 1-8-1 Inohana, 260-8673, Chuo-ku, Chiba, Japan;Medical Mycology Research Center, Chiba University, 1-8-1 Inohana, 260-8673, Chuo-ku, Chiba, Japan;Molecular Chirality Research Center, Chiba University, 1-33 Yayoi-cho, 263-8522, Inage-ku, Chiba, Japan;

关键词: Transcriptome;    RNA-seq;    Aspergillus fumigatus;    Environmental stress;    ESR;   

DOI  :  10.1186/s12864-017-4316-z

 received in 2017-09-05, accepted in 2017-11-17,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundAspergillus fumigatus is a human fungal pathogen that causes aspergillosis in immunocompromised hosts. A. fumigatus is believed to be exposed to diverse environmental stresses in the host cells. The adaptation mechanisms are critical for infections in human bodies. Transcriptional networks in response to diverse environmental challenges remain to be elucidated. To gain insights into the adaptation to environmental stresses in A. fumigatus mycelia, we conducted time series transcriptome analyses.ResultsWith the aid of RNA-seq, we explored the global gene expression profiles of mycelia in A. fumigatus upon exposure to diverse environmental changes, including heat, superoxide, and osmotic stresses. From the perspective of global transcriptomes, transient responses to superoxide and osmotic stresses were observed while responses to heat stresses were gradual. We identified the stress-responsive genes for particular stresses, and the 266 genes whose expression levels drastically fluctuated upon exposure to all tested stresses. Among these, the 77 environmental stress response genes are conserved in S. cerevisiae, suggesting that these genes might be more general prerequisites for adaptation to environmental stresses. Finally, we revealed the strong correlations among expression profiles of genes related to ‘rRNA processing’.ConclusionsThe time series transcriptome analysis revealed the stress-responsive genes underlying the adaptation mechanisms in A. fumigatus mycelia. These results will shed light on the regulatory networks underpinning the adaptation of the filamentous fungi.

【 授权许可】

CC BY   
© The Author(s). 2017

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