Differential mucosal expression of Th17-related genes between the inflamed colon and ileum of patients with inflammatory bowel disease

BMC Immunology

Research Article

Jo Vandesompele¹  Harald Peeters²  Sara Bogaert²  Kim Olievier²  Debby Laukens²  Martine De Vos²  Dirk Elewaut³  Lode Melis³  Gust Verbruggen³  Nico Boon⁴ 

[1] Center for Medical Genetics, Ghent University, Ghent, Belgium;Biogazelle, Ghent, Belgium;Department of Gastroenterology, Ghent University, Ghent, Belgium;Department of Rheumatology, Ghent University, Ghent, Belgium;The Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Ghent, Belgium;

关键词: Inflammatory Bowel Disease;    Ulcerative Colitis;    Th17 Cell;    Inflammatory Bowel Disease Patient;    Ulcerative Colitis Patient;   

DOI  :  10.1186/1471-2172-11-61

 received in 2010-05-21, accepted in 2010-12-13,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundImmunological and genetic findings implicate Th17 effector cytokines in the pathogenesis of inflammatory bowel disease (IBD). Expression of Th17 pathway-associated genes is mainly studied in colonic disease. The present study assessed the mRNA expression levels of Th17 effector cytokines (IL17A, IL17F, IL21, IL22 and IL26) and genes involved in differentiation (IL6, IL1B, TGFB1, IL23A and STAT3) and recruitment of Th17 cells (CCR6 and CCL20) by quantitative real-time PCR analysis of colonic and ileal biopsies from 22 healthy control subjects, 26 patients with Crohn's disease (CD) and 12 patients with ulcerative colitis (UC). Inflammation was quantified by measuring expression of the inflammatory mediators IL8 and TNF.ResultsEvaluation of mRNA expression levels in colonic and ileal control samples revealed that TNF, TGFB1, STAT3 and CCR6 were expressed at higher levels in the ileum than in the colon. Expression of all the Th17 pathway-associated genes was increased in inflamed colonic samples. The increased expression of these genes was predominantly observed in samples from UC patients and was associated with more intense inflammation. Although increased expression of IL17A, IL17F, IL21 and IL26 was detected in inflamed ileal samples, expression of the indispensable Th17 cell differentiation factors TGFB1 and IL23A, the signaling molecule STAT3 and the Th17 recruitment factors CCR6 and CCL20 were unchanged.ConclusionsOur findings suggest that immune regulation is different in colonic and ileal disease, which might have important consequences for therapeutic intervention.

【 授权许可】

Unknown   
© Bogaert et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.