Cardiovascular magnetic resonance feature tracking in small animals – a preliminary study on reproducibility and sample size calculation

BMC Medical Imaging

Research Article

Ulrich Kintscher¹  Remigijus Zaliunas²  Tomas Lapinskas³  Sarah Jeuthe⁴  Heike Meyborg⁵  Seyedeh Mahsa Zamani⁵  Jana Grune⁶  Rolf Gebker⁷  Sebastian Kelle⁷  Burkert Pieske⁷  Philipp Stawowy⁷  Daniel Messroghli⁸ 

[1] Center for Cardiovascular Research, Institute of Pharmacology, Charité-Universitätsmedizin Berlin, Berlin, Germany;DZHK (German Centre for Cardiovascular Research), Partner Site Berlin, Berlin, Germany;Department of Cardiology, Medical Academy, Lithuanian University of Health Sciences, Eiveniu Street 2, LT-50161, Kaunas, Lithuania;Department of Cardiology, Medical Academy, Lithuanian University of Health Sciences, Eiveniu Street 2, LT-50161, Kaunas, Lithuania;Department of Internal Medicine / Cardiology, Deutsches Herzzentrum Berlin, Augustenburger Platz 1, 13353, Berlin, Germany;DZHK (German Centre for Cardiovascular Research), Partner Site Berlin, Berlin, Germany;Department of Internal Medicine / Cardiology, Charité-Universitätsmedizin Berlin, Berlin, Germany;DZHK (German Centre for Cardiovascular Research), Partner Site Berlin, Berlin, Germany;Department of Internal Medicine / Cardiology, Deutsches Herzzentrum Berlin, Augustenburger Platz 1, 13353, Berlin, Germany;Department of Internal Medicine / Cardiology, Deutsches Herzzentrum Berlin, Augustenburger Platz 1, 13353, Berlin, Germany;Center for Cardiovascular Research, Institute of Pharmacology, Charité-Universitätsmedizin Berlin, Berlin, Germany;DZHK (German Centre for Cardiovascular Research), Partner Site Berlin, Berlin, Germany;Department of Internal Medicine / Cardiology, Deutsches Herzzentrum Berlin, Augustenburger Platz 1, 13353, Berlin, Germany;DZHK (German Centre for Cardiovascular Research), Partner Site Berlin, Berlin, Germany;Department of Internal Medicine / Cardiology, Deutsches Herzzentrum Berlin, Augustenburger Platz 1, 13353, Berlin, Germany;Department of Internal Medicine / Cardiology, Charité-Universitätsmedizin Berlin, Berlin, Germany;DZHK (German Centre for Cardiovascular Research), Partner Site Berlin, Berlin, Germany;

关键词: Cardiovascular magnetic resonance;    Feature tracking;    Myocardial strain;    Small animal model;    Reproducibility;   

DOI  :  10.1186/s12880-017-0223-7

 received in 2017-03-11, accepted in 2017-08-17,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundCardiovascular magnetic resonance feature tracking (CMR-FT) is a novel tissue tracking technique developed for noninvasive assessment of myocardial motion and deformation. This preliminary study aimed to evaluate the observer’s reproducibility of CMR-FT in a small animal (mouse) model and define sample size calculation for future trials.MethodsSix C57BL/6 J mice were selected from the ongoing experimental mouse model onsite and underwent CMR with a 3 Tesla small animal MRI scanner. Myocardial deformation was analyzed using dedicated software (TomTec, Germany) by two observers. Left ventricular (LV) longitudinal, circumferential and radial strain (EllLAX, EccSAX and ErrSAX) were calculated. To assess intra-observer agreement data analysis was repeated after 4 weeks. The sample size required to detect a relative change in strain was calculated.ResultsIn general, EccSAX and EllLAX demonstrated highest inter-observer reproducibility (ICC 0.79 (0.46–0.91) and 0.73 (0.56–0.83) EccSAX and EllLAX respectively). In contrast, at the intra-observer level EllLAX was more reproducible than EccSAX (ICC 0.83 (0.73–0.90) and 0.74 (0.49–0.87) EllLAX and EccSAX respectively). The reproducibility of ErrSAX was weak at both observer levels. Preliminary sample size calculation showed that a small study sample (e.g. ten animals to detect a relative 10% change in EccSAX) could be sufficient to detect changes if parameter variability is low.ConclusionsThis pilot study demonstrates good to excellent inter- and intra-observer reproducibility of CMR-FT technique in small animal model. The most reproducible measures are global circumferential and global longitudinal strain, whereas reproducibility of radial strain is weak. Furthermore, sample size calculation demonstrates that a small number of animals could be sufficient for future trials.

【 授权许可】

CC BY   
© The Author(s). 2017