Analysis for genotyping Duffy blood group in inhabitants of Sudan, the Fourth Cataract of the Nile

Malaria Journal

Research

Milosz Parczewski¹  Arleta Drozd²  Mariusz Kaczmarczyk³  Andrzej Ciechanowicz³  Miroslaw Parafiniuk⁴  Oktawian Knap⁵  Agnieszka Kempińska-Podhorodecka⁶ 

[1] Chair and Clinic of Infectious Diseases, Pomeranian Medical University, Arkońska 4, 71-455, Szczecin, Poland;Department of Biochemistry and Human Nutrition, Pomeranian Medical University, Broniewskiego 24, 71-460, Szczecin, Poland;Department of Clinical and Molecular Biochemistry, Pomeranian Medical University, Powstancow Wlkp 72, 70-111, Szczecin, Poland;Department of Forensic Medicine, Pomeranian Medical University, Powstancow Wlkp 72, 70-111, Szczecin, Poland;Independent Laboratory of Disaster Medicine, Pomeranian Medical University, Rybacka 1, 70-204, Szczecin, Poland;Medical Biology Laboratory, Pomeranian Medical University, Powstancow Wlkp 72, 70-111, Szczecin, Poland;

关键词: Duffy blood group;    Malaria;    Sudanese Arabs;    Shagia;    Manasir;    SNP polymorphism;    Real-Time PCR;   

DOI  :  10.1186/1475-2875-11-115

 received in 2011-11-30, accepted in 2012-04-17,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundGenetic polymophisms of the Duffy antigen receptor for the chemokines (DARC) gene successfully protected against blood stage infection by Plasmodium vivax infection. The Fy (a-, b-) phenotype is predominant among African populations, particularly those originating from West Africa, and it is rare among non-African populations. The aim of this study was to analyse the frequency of four Duffy blood groups based on SNPs (T-33C, G125A, G298A and C5411T) in two local tribes of Sudanese Arabs, the Shagia and Manasir, which are both from the region of the Fourth Nile cataract in Sudan.MethodsAn analysis of polymorphisms was performed on 217 individuals (126 representatives of the Shagia tribe and 91 of the Manasir). Real-time PCR and TaqMan Genotyping Assays were used to study the prevalence of alleles and genotypes.ResultsThe analysis of allelic and genotype frequency in the T-33C polymorphisms demonstrated a significant dominance of the C allele and CC genotype (OR = 0.53 [0.32-0.88]; p = 0.02) in both tribes. The G125A polymorphism is associated with phenotype Fy(a-, b-) and was identified in 83% of Shagia and 77% of Manasir. With regard to G298A polymorphisms, the genotype frequencies were different between the tribes (p = 0,002) and no single AA homozygote was found. Based on four SNPs examined, 20 combinations of genotypes for the Shagia and Manasir tribes were determined. The genotype CC/AA/GG/CT occurred most often in Shagia tribe (45.9%) but was rare in the Manasir tribe (6.6%) (p < 0.001 Shagia versus Manasir). The FY*AES allele was identified in both analysed tribes. The presence of individuals with the FY*A/FY*A genotype was demonstrated only in the Shagia tribe.ConclusionThis is probably the first report showing genotypically Duffy-negative people who carry both FY*BES and FY*AES . The identification of the FY*AES allele in both tribes may be due to admixture of the non-African genetic background. Taken as a whole, allele and genotype frequencies between the Shagia and the Manasir were statistically different. However, the presence of individuals with the FY*A/FY*A genotype was demonstrated only in the Shagia tribe.

【 授权许可】

CC BY   
© Kempińska-Podhorodecka et al; licensee BioMed Central Ltd. 2012