Bacterial community characterization of water and intestine of the shrimp Litopenaeus stylirostris in a biofloc system

BMC Microbiology

Research Article

David Piquemal¹  Florian Noguier¹  Fabien Pierrat¹  Bénédicte Lorgeoux²  Kevin Magré²  Denis Saulnier²  Yannick Gueguen³  Emilie Cardona⁴ 

[1] ACOBIOM, 1682 rue de la Valsière, Cap Delta - CS77394, 34184, Montpellier Cedex 4, France;Ifremer, UMR 241 EIO, UPF-ILM-IRD, Labex Corail, B.P. 7004, 98719, Taravao, Tahiti, French Polynesia;Ifremer, UMR 241 EIO, UPF-ILM-IRD, Labex Corail, B.P. 7004, 98719, Taravao, Tahiti, French Polynesia;Ifremer, UMR 5244 IHPE, UPVD, CNRS, Université de Montpellier, F-34095, Montpellier, France;Ifremer, UMR 241 EIO, UPF-ILM-IRD, Labex Corail, B.P. 7004, 98719, Taravao, Tahiti, French Polynesia;Ifremer, Unité de recherche Lagons, Ecosystèmes et Aquaculture Durable en Nouvelle Calédonie, Nouméa, New Caledonia;

关键词: Shrimp;    Biofloc;    Clear sea water;    Bacterial communities;    Culture environment;    Microbiota;   

DOI  :  10.1186/s12866-016-0770-z

 received in 2015-10-03, accepted in 2016-07-12,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundBiofloc technology (BFT), a rearing method with little or no water exchange, is gaining popularity in aquaculture. In the water column, such systems develop conglomerates of microbes, algae and protozoa, together with detritus and dead organic particles. The intensive microbial community presents in these systems can be used as a pond water quality treatment system, and the microbial protein can serve as a feed additive. The current problem with BFT is the difficulty of controlling its bacterial community composition for both optimal water quality and optimal shrimp health. The main objective of the present study was to investigate microbial diversity of samples obtained from different culture environments (Biofloc technology and clear seawater) as well as from the intestines of shrimp reared in both environments through high-throughput sequencing technology.ResultsAnalyses of the bacterial community identified in water from BFT and “clear seawater” (CW) systems (control) containing the shrimp Litopenaeus stylirostris revealed large differences in the frequency distribution of operational taxonomic units (OTUs). Four out of the five most dominant bacterial communities were different in both culture methods. Bacteria found in great abundance in BFT have two principal characteristics: the need for an organic substrate or nitrogen sources to grow and the capacity to attach to surfaces and co-aggregate. A correlation was found between bacteria groups and physicochemical and biological parameters measured in rearing tanks. Moreover, rearing-water bacterial communities influenced the microbiota of shrimp. Indeed, the biofloc environment modified the shrimp intestine microbiota, as the low level (27 %) of similarity between intestinal bacterial communities from the two treatments.ConclusionThis study provides the first information describing the complex biofloc microbial community, which can help to understand the environment-microbiota-host relationship in this rearing system.

【 授权许可】

CC BY   
© The Author(s). 2016