Dynasore - not just a dynamin inhibitor

Cell Communication and Signaling

Review

Giulio Preta¹  James G Cronin¹  I Martin Sheldon¹ 

[1] Institute of Life Science, College of Medicine, Swansea University, SA2 8PP, Swansea, UK;

关键词: Dynasore;    GTPase;    Dynamin;    Endocytosis;    Cholesterol;    Lipid rafts;   

DOI  :  10.1186/s12964-015-0102-1

 received in 2015-02-04, accepted in 2015-03-26,  发布年份 2015

来源: Springer

【 摘 要 】

Dynamin is a GTPase protein that is essential for membrane fission during clathrin-mediated endocytosis in eukaryotic cells. Dynasore is a GTPase inhibitor that rapidly and reversibly inhibits dynamin activity, which prevents endocytosis. However, comparison between cells treated with dynasore and RNA interference of genes encoding dynamin, reveals evidence that dynasore reduces labile cholesterol in the plasma membrane, and disrupts lipid raft organization, in a dynamin-independent manner. To explore the role of dynamin it is important to use multiple dynamin inhibitors, alongside the use of dynamin mutants and RNA interference targeting genes encoding dynamin. On the other hand, dynasore provides an interesting tool to explore the regulation of cholesterol in plasma membranes.

【 授权许可】

Unknown   
© Preta et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.