Diversity of lactic acid bacteria of the bioethanol process

BMC Microbiology

Research Article

Vasco Azevedo¹  João LS Moreira¹  Alvaro C Nunes¹  Anderson Miyoshi¹  Billy M dos Santos²  Marcos Antonio de Morais²  Ana Paula B Moreira³  Fabiano L Thompson³  Brigida TL Lucena⁴ 

[1] Department of General Biology, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil;Department of Genetics, Federal University of Pernambuco (UFPE), Recife, Brazil;Institute of Biology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil;Institute of Biology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil;Department of Genetics, Federal University of Pernambuco (UFPE), Recife, Brazil;

关键词: Lactic Acid Bacterium;    Sugarcane Juice;    Lactic Acid Bacterium Isolate;    Lactic Acid Bacterium Species;    Sugar Cane Juice;   

DOI  :  10.1186/1471-2180-10-298

 received in 2010-07-31, accepted in 2010-11-23,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundBacteria may compete with yeast for nutrients during bioethanol production process, potentially causing economic losses. This is the first study aiming at the quantification and identification of Lactic Acid Bacteria (LAB) present in the bioethanol industrial processes in different distilleries of Brazil.ResultsA total of 489 LAB isolates were obtained from four distilleries in 2007 and 2008. The abundance of LAB in the fermentation tanks varied between 6.0 × 105 and 8.9 × 108 CFUs/mL. Crude sugar cane juice contained 7.4 × 107 to 6.0 × 108 LAB CFUs. Most of the LAB isolates belonged to the genus Lactobacillus according to rRNA operon enzyme restriction profiles. A variety of Lactobacillus species occurred throughout the bioethanol process, but the most frequently found species towards the end of the harvest season were L. fermentum and L. vini. The different rep-PCR patterns indicate the co-occurrence of distinct populations of the species L. fermentum and L. vini, suggesting a great intraspecific diversity. Representative isolates of both species had the ability to grow in medium containing up to 10% ethanol, suggesting selection of ethanol tolerant bacteria throughout the process.ConclusionsThis study served as a first survey of the LAB diversity in the bioethanol process in Brazil. The abundance and diversity of LAB suggest that they have a significant impact in the bioethanol process.

【 授权许可】

Unknown   
© Lucena et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.