Microglial responses around intrinsic CNS neurons are correlated with axonal regeneration

BMC Neuroscience

Research Article

Koujiro Tohyama¹  Bernadette ZY Wong²  Patrick N Anderson²  Bahman N Shokouhi²  Samir Siddiqui²  A Robert Lieberman²  Gregor Campbell² 

[1] Centre for Electron Microscopy and Bioimaging Research, Laboratory for Nano-Neuroanatomy, Iwate Medical University School of Medicine, 19-1 Uchimaru, 020-8505, Morioka, Iwate, Japan;Department of Cell and Developmental Biology, University College London, Gower Street, WC1E 6BT, London, UK;

关键词: Motor Cortex;    Microglial Activation;    Axonal Regeneration;    Thalamic Reticular Nucleus;    Retrograde Labelling;   

DOI  :  10.1186/1471-2202-11-13

 received in 2009-08-12, accepted in 2010-02-05,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundMicroglia/macrophages and lymphocytes (T-cells) accumulate around motor and primary sensory neurons that are regenerating axons but there is little or no microglial activation or T-cell accumulation around axotomised intrinsic CNS neurons, which do not normally regenerate axons. We aimed to establish whether there was an inflammatory response around the perikarya of CNS neurons that were induced to regenerate axons through a peripheral nerve graft.ResultsWhen neurons of the thalamic reticular nucleus (TRN) and red nucleus were induced to regenerate axons along peripheral nerve grafts, a marked microglial response was found around their cell bodies, including the partial enwrapping of some regenerating neurons. T-cells were found amongst regenerating TRN neurons but not rubrospinal neurons. Axotomy alone or insertion of freeze-killed nerve grafts did not induce a similar perineuronal inflammation. Nerve grafts in the corticospinal tracts did not induce axonal regeneration or a microglial or T-cell response in the motor cortex.ConclusionsThese results strengthen the evidence that perineuronal microglial accumulation (but not T-cell accumulation) is involved in axonal regeneration by intrinsic CNS and other neurons.

【 授权许可】

Unknown   
© Shokouhi et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.