Decreased level of recent thymic emigrants in CD4+ and CD8+T cells from CML patients

Journal of Translational Medicine

Research

Christian A Schmidt¹  Grzegorz K Przybylski²  Xin Du³  Lijian Yang⁴  Bo Li⁴  Xiuli Wu⁴  Qingsong Yin⁴  Shaohua Chen⁴  Suxia Geng⁵  Yangqiu Li⁶ 

[1] Department of Hematology and Oncology, Ernst-Moritz-Arndt University Greifswald, 17487, Greifswald, Germany;Department of Hematology and Oncology, Ernst-Moritz-Arndt University Greifswald, 17487, Greifswald, Germany;Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland;Department of Hematology, Guangdong Province People's Hospital, 510080, Guangzhou, China;Institute of Hematology, Medical College, Jinan University, 510632, Guangzhou, China;Institute of Hematology, Medical College, Jinan University, 510632, Guangzhou, China;Department of Hematology, Guangdong Province People's Hospital, 510080, Guangzhou, China;Institute of Hematology, Medical College, Jinan University, 510632, Guangzhou, China;Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, 510632, Guangzhou, China;

关键词: Chronic Myeloid Leukemia;    Chronic Myeloid Leukemia Patient;    Thymic Output;    Boster Biological Technology;    Recent Thymic Emigrant;   

DOI  :  10.1186/1479-5876-8-47

 received in 2009-11-28, accepted in 2010-05-14,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundT-cell immunodeficiency is a common feature in cancer patients, which may relate to initiation and development of tumor. Based on our previous finding, to further characterize the immune status, T cell proliferative history was analyzed in CD4+ and CD8+ T cells from chronic myeloid leukemia (CML) patients.MethodsQuantitative analysis of δRec-ψJα signal joint T cell receptor excision circles (sjTRECs) was performed in PBMCs, CD3+, CD4+ and CD8+T cells by real-time PCR, and the analysis of 23 TRBV-D1 sjTRECs was performed by semi-nested PCR. Forty eight CML cases in chronic phase (CML-CP) were selected for this study and 17 healthy individuals served as controls.ResultsThe levels of δRec-ψJα sjTRECs in PBMCs, CD3+, CD4+, and CD8+ T cells were significantly decreased in CML patients, compared with control groups. Moreover, the numbers of detectable TRBV subfamily sjTRECs, as well as the frequency of particular TRBV-BD 1 sjTRECs in patients with CML were significantly lower than those from healthy individuals.ConclusionsWe observed decreased levels of recent thymic emigrants in CD4+ and CD8+ T cells that may underlay the persistent immunodeficiency in CML patients.

【 授权许可】

CC BY   
© Li et al; licensee BioMed Central Ltd. 2010