Antiplasmodial activity of Indigofera spicata root extract against Plasmodium berghei infection in mice

Malaria Journal

Research

Abyot Endale Gurmu¹  Eshetie Melese Birru²  Mestayet Geta² 

[1] Department of Pharmacognosy, School of Pharmacy, College of Medicine and Health Sciences, University of Gondar, P.O.box 196, Chechela Street, Lideta Subcity kebele 16, Gondar, Ethiopia;Department of Pharmacology, School of Pharmacy, College of Medicine and Health Sciences, University of Gondar, P.O.box 196, Chechela Street, Lideta Subcity kebele 16, Gondar, Ethiopia;

关键词: Malaria;    Herbal medicine;    Indigofera spicata;    Plasmodium berghei;    In vivo;   

DOI  :  10.1186/s12936-017-1853-5

 received in 2017-03-07, accepted in 2017-05-09,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundIn addition to pharmacovigilance and pharmaco-economic concerns, resistance to anti-malarial medicines has been documented in all classes of anti-malarials and this is further worsened by resistance to common insecticides by malaria vector, which is a major threat to malaria control. As a means of facing the challenges of searching for new anti-malarial agents, the current study focused on evaluation of anti-malarial activity of root extract of Indigofera spicata.MethodsChloroquine-sensitive rodent malaria parasite, Plasmodium berghei (ANKA strain) was used to infect the Swiss Albino mice in 4-day suppressive and curative models. The crude hydromethanolic root extract of I. spicata at 200, 400 and 600 mg/kg doses was administered to a group of five mice. Important parameters, such as level of parasitaemia, packed cell volume (PCV), survival time, and body weight were determined and the significance of the differences between mean values of the five groups was analysed by one-way ANOVA followed by post hoc Tukey’s Multiple Comparison test.ResultsIn both the suppressive and curative models, 400 and 600 mg/kg doses of the extract suppressed the level of parasitaemia significantly (p < 0.001) compared to the vehicle-treated groups, 34.93 and 53.42%, respectively. However, only the mice which were treated with the 600 mg/kg dose of the extract had significant difference in their mean survival time. In other parameters, namely PCV and mean body weight, there was no statistically significant difference between the extract-treated groups when compared to the negative control.ConclusionsThis study revealed that the root extract of I. spicata possesses anti-malarial activity and necessitates further scientific validation.

【 授权许可】

CC BY   
© The Author(s) 2017