Genome-wide identification and characterization of auxin response factor (ARF) family genes related to flower and fruit development in papaya (Carica papaya L.)

BMC Genomics

Research Article

Wanhuang Lin¹  Kaidong Liu²  Xiaolin Zheng³  Yanjun Yang⁴  Chenjia Shen⁴  Haili Li⁵  Changchun Yuan⁵ 

[1] College of Bioscience and Technology, Hunan Agricultural University, 410128, Changsha, Hunan, China;College of Bioscience and Technology, Hunan Agricultural University, 410128, Changsha, Hunan, China;Life Science and Technology School, Lingnan Normal University, 524048, Zhanjiang, Guangdong, China;College of Food Science and Biotechnology, Zhejiang Gongshang University, 310035, Hangzhou, China;College of Life and Environmental Sciences, Hangzhou Normal University, 310036, Hangzhou, China;Life Science and Technology School, Lingnan Normal University, 524048, Zhanjiang, Guangdong, China;

关键词: Auxin;    Auxin response factor;    Papaya;    Developmental process;    Fruit ripening;   

DOI  :  10.1186/s12864-015-2182-0

 received in 2015-06-05, accepted in 2015-10-30,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundAuxin and auxin signaling are involved in a series of developmental processes in plants. Auxin Response Factors (ARFs) is reported to modulate the expression of target genes by binding to auxin response elements (AuxREs) and influence the transcriptional activation of down-stream target genes. However, how ARF genes function in flower development and fruit ripening of papaya (Carica papaya L.) is largely unknown. In this study, a comprehensive characterization and expression profiling analysis of 11 C. papaya ARF (CpARF) genes was performed using the newly updated papaya reference genome data.ResultsWe analyzed CpARF expression patterns at different developmental stages. CpARF1, CpARF2, CpARF4, CpARF5, and CpARF10 showed the highest expression at the initial stage of flower development, but decreased during the following developmental stages. CpARF6 expression increased during the developmental process and reached its peak level at the final stage of flower development. The expression of CpARF1 increased significantly during the fruit ripening stages. Many AuxREs were included in the promoters of two ethylene signaling genes (CpETR1 and CpETR2) and three ethylene-synthesis-related genes (CpACS1, CpACS2, and CpACO1), suggesting that CpARFs might be involved in fruit ripening via the regulation of ethylene signaling.ConclusionsOur study provided comprehensive information on ARF family in papaya, including gene structures, chromosome locations, phylogenetic relationships, and expression patterns. The involvement of CpARF gene expression changes in flower and fruit development allowed us to understand the role of ARF-mediated auxin signaling in the maturation of reproductive organs in papaya.

【 授权许可】

CC BY   
© Liu et al. 2015