Systemic immune response and virus persistence after foot-and-mouth disease virus infection of naïve cattle and cattle vaccinated with a homologous adenovirus-vectored vaccine

BMC Veterinary Research

Research Article

Mary A. Kenney¹  Jonathan Arzt¹  Ethan J. Hartwig¹  George R. Smoliga¹  Luis L. Rodriguez¹  Juan M. Pacheco¹  William T. Golde¹  Steven I. Rekant²  Carolina Stenfeldt²  Michael Eschbaumer² 

[1] United States Department of Agriculture (USDA), Plum Island Animal Disease Center (PIADC), Foreign Animal Disease Research Unit (FADRU), Agricultural Research Service (ARS), P.O. Box 848, 11944, Greenport, NY, USA;United States Department of Agriculture (USDA), Plum Island Animal Disease Center (PIADC), Foreign Animal Disease Research Unit (FADRU), Agricultural Research Service (ARS), P.O. Box 848, 11944, Greenport, NY, USA;Oak Ridge Institute for Science and Education, PIADC Research Participation Program, Oak Ridge, TN, USA;

关键词: FMDV;    Vaccination;    Persistence;    Carrier;    Flow cytometry;    Lymphopenia;    Interferon;    ELISA;    ELISPOT;   

DOI  :  10.1186/s12917-016-0838-x

 received in 2016-01-05, accepted in 2016-09-10,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundIn order to investigate host factors associated with the establishment of persistent foot-and-mouth disease virus (FMDV) infection, the systemic response to vaccination and challenge was studied in 47 steers. Eighteen steers that had received a recombinant FMDV A vaccine 2 weeks earlier and 29 non-vaccinated steers were challenged by intra-nasopharyngeal deposition of FMDV A24. For up to 35 days after challenge, host factors including complete blood counts with T lymphocyte subsets, type I/III interferon (IFN) activity, neutralizing and total FMDV-specific antibody titers in serum, as well as antibody-secreting cells (in 6 non-vaccinated animals) were characterized in the context of viral infection dynamics.ResultsVaccination generally induced a strong antibody response. There was a transient peak of FMDV-specific serum IgM in non-vaccinated animals after challenge, while IgM levels in vaccinated animals did not increase further. Both groups had a lasting increase of specific IgG and neutralizing antibody after challenge.Substantial systemic IFN activity in non-vaccinated animals coincided with viremia, and no IFN or viremia was detected in vaccinated animals. After challenge, circulating lymphocytes decreased in non-vaccinated animals, coincident with viremia, IFN activity, and clinical disease, whereas lymphocyte and monocyte counts in vaccinated animals were unaffected by vaccination but transiently increased after challenge. The CD4+/CD8+ T cell ratio in non-vaccinated animals increased during acute infection, driven by an absolute decrease of CD8+ cells.ConclusionsThe incidence of FMDV persistence was 61.5 % in non-vaccinated and 54.5 % in vaccinated animals. Overall, the systemic factors examined were not associated with the FMDV carrier/non-carrier divergence; however, significant differences were identified between responses of non-vaccinated and vaccinated cattle.

【 授权许可】

CC BY   
© The Author(s). 2016