Characterization of phosphorus-regulated miR399 and miR827 and their isomirs in barley under phosphorus-sufficient and phosphorus-deficient conditions

BMC Plant Biology

Research Article

Bu-Jun Shi¹  Peter Langridge¹  Michael Hackenberg²  Perry Gustafson³ 

[1] Australian Centre for Plant Functional Genomics, The University of Adelaide, 5064, Urrbrae, South Australia, Australia;Computational Genomics and Bioinformatics Group, Genetics Department, University of Granada, 18071, Granada, Spain;USDA-ARS, University of Missouri, 206 Curtis Hall, 65211-7020, Columbia, MO, USA;

关键词: Barley;    Phosphorus;    miR399;    miR827;    Isomir;    Antisense;    Targets;    Differential expression;   

DOI  :  10.1186/1471-2229-13-214

 received in 2013-08-23, accepted in 2013-11-16,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundmiR399 and miR827 are both involved in conserved phosphorus (P) deficiency signalling pathways. miR399 targets the PHO2 gene encoding E2 enzyme that negatively regulates phosphate uptake and root-to-shoot allocation, while miR827 targets SPX-domain-containing genes that negatively regulate other P-responsive genes. However, the response of miR399 and miR827 to P conditions in barley has not been investigated.ResultsIn this study, we investigated the expression profiles of miR399 and miR827 in barley (Hordeum vulagre L.) under P-deficient and P-sufficient conditions. We identified 10 members of the miR399 family and one miR827 gene in barley, all of which were significantly up-regulated under deficient P. In addition, we found many isomirs of the miR399 family and miR827, most of which were also significantly up-regulated under deficient P. Several isomirs of miR399 members were found to be able to cleave their predicted targets in vivo. Surprisingly, a few small RNAs (sRNAs) derived from the single-stranded loops of the hairpin structures of MIR399b and MIR399e-1 were also found to be able to cleave their predicted targets in vivo. Many antisense sRNAs of miR399 and a few for miR827 were also detected, but they did not seem to be regulated by P. Intriguingly, the lowest expressed member, hvu-miR399k, had four-fold more antisense sRNAs than sense sRNAs, and furthermore under P sufficiency, the antisense sRNAs are more frequent than the sense sRNAs. We identified a potential regulatory network among miR399, its target HvPHO2 and target mimics HvIPS1 and HvIPS2 in barley under P-deficient and P-sufficient conditions.ConclusionsOur data provide an important insight into the mechanistic regulation and function of miR399, miR827 and their isomirs in barley under different P conditions.

【 授权许可】

Unknown   
© Hackenberg et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.