Deregulation of manganese superoxide dismutase (SOD2) expression and lymph node metastasis in tongue squamous cell carcinoma

BMC Cancer

Research Article

Hui Ye¹  Fei Shi²  David L Crowe³  Xiqiang Liu⁴  Xiaofeng Zhou⁵  Tianwei Yu⁶  Antonia Kolokythas⁷  Anxun Wang⁸  Shihu Sheng⁸  Corrado Rubini⁹  Lorenzo Lo Muzio^1,10 

[1] Center for Molecular Biology of Oral Diseases, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA;Center for Molecular Biology of Oral Diseases, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA;Division of Epidemiology and Biostatistics, School of Public Health, University of Illinois at Chicago, Chicago, IL, USA;Center for Molecular Biology of Oral Diseases, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA;Graduate College, UIC Cancer Center, University of Illinois at Chicago, Chicago, IL, USA;Center for Molecular Biology of Oral Diseases, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA;Research Institute & the Affiliated Hospital of Stomatology, Sun Yat-Sen University, Guangzhou, China;Center for Molecular Biology of Oral Diseases, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA;Research Institute & the Affiliated Hospital of Stomatology, Sun Yat-Sen University, Guangzhou, China;Graduate College, UIC Cancer Center, University of Illinois at Chicago, Chicago, IL, USA;Department of Biostatistics and Bioinformatics, Rollins School of Public Health, Emory University, Atlanta, GA, USA;Department of Oral and Maxillofacial Surgery, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA;Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China;Department of Pathology, Faculty of Medicine, Marche Polytechnic University, Ancona, Italy;Department of Surgical Sciences, Faculty of Medicine, School of Dentistry, University of Foggia, Foggia, Italy;

关键词: Oral Squamous Cell Carcinoma;    SOD2 Level;    Oral Squamous Cell Carcinoma;    Tongue Squamous Cell Carcinoma;    SOD2 Expression;   

DOI  :  10.1186/1471-2407-10-365

 received in 2009-07-16, accepted in 2010-07-09,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundLymph node metastasis is a critical event in the progression of tongue squamous cell carcinoma (TSCC). The identification of biomarkers associated with the metastatic process would provide critical prognostic information to facilitate clinical decision making. Previous studies showed that deregulation of manganese superoxide dismutase (SOD2) expression is a frequent event in TSCC and may be associated with enhanced cell invasion. The purpose of this study is to further evaluate whether the expression level of SOD2 is correlated with the metastatic status in TSCC patients.MethodsWe first examined the SOD2 expression at mRNA level on 53 TSCC and 22 normal control samples based on pooled-analysis of existing microarray datasets. To confirm our observations, we examined the expression of SOD2 at protein level on an additional TSCC patient cohort (n = 100), as well as 31 premalignant dysplasias, 15 normal tongue mucosa, and 32 lymph node metastatic diseases by immunohistochemistry (IHC).ResultsThe SOD2 mRNA level in primary TSCC tissue is reversely correlated with lymph node metastasis in the first TSCC patient cohort. The SOD2 protein level in primary TSCC tissue is also reversely correlated with lymph node metastasis in the second TSCC patient cohort. Deregulation of SOD2 expression is a common event in TSCC and appears to be associated with disease progression. Statistical analysis revealed that the reduced SOD2 expression in primary tumor tissue is associated with lymph node metastasis in both TSCC patient cohorts examined.ConclusionsOur study suggested that the deregulation of SOD2 in TSCC has potential predictive values for lymph node metastasis, and may serve as a therapeutic target for patients at risk of metastasis.

【 授权许可】

Unknown   
© Liu et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.