Mitf-Mdel, a novel melanocyte/melanoma-specific isoform of microphthalmia-associated transcription factor-M, as a candidate biomarker for melanoma

BMC Medicine

Research Article

Suhu Liu¹  Soroosh Radfar²  Adam I Riker³  Hung T Khong⁴  Yixiang Wang⁵ 

[1] Dana-Farber Cancer Institute, Boston, MA, USA;Department of Internal Medicine, Hematology section, Yale University School of Medicine, PO Box 208021, 333 Cedar Street, New Haven, CT, USA;Department of Surgery, Ochsner Cancer Institute, 1514 Jefferson Highway, BH334, 70121, New Orleans, LA, USA;Mitchell Cancer Institute, University of South Alabama, 1660 Springhill Avenue, 36604, Mobile, AL, USA;Research Laboratory of Oral and Maxillofacial Surgery, Peking University School of Stomatology, Beijing, China;

关键词: Melanoma;    Reverse Transcription Polymerase Chain Reaction;    Melanoma Cell Line;    Human Melanoma Cell Line;    Quantitative Reverse Transcription Polymerase Chain Reaction;   

DOI  :  10.1186/1741-7015-8-14

 received in 2009-12-17, accepted in 2010-02-17,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundMelanoma incidence is on the rise and advanced melanoma carries an extremely poor prognosis. Treatment options, including chemotherapy and immunotherapy, are limited and offer low response rates and transient efficacy. Thus, identification of new melanocyte/melanoma antigens that serve as potential novel candidate biomarkers in melanoma is an important area for investigation.MethodsFull length MITF-M and its splice variant cDNA were cloned from human melanoma cell line 624 mel by reverse transcription polymerase chain reaction (RT-PCR). Expression was investigated using regular and quantitative RT-PCR in three normal melanocytes (NHEM), 31 melanoma cell lines, 21 frozen melanoma tissue samples, 18 blood samples (pheripheral blood mononuclear cell; PBMC) from healthy donors and 12 non-melanoma cancer cell lines, including three breast, five glioma, one sarcoma, two kidney and one ovarian cancer cell lines.ResultsA novel splice variant of MITF-M, which we named MITF-Mdel, was identified. The predicted MITF-Mdel protein contains two in frame deletions, 56- and 6- amino acid deletions in exon 2 (from V32 to E87) and exon 6 (from A187 to T192), respectively. MITF-Mdel was widely expressed in melanocytes, melanoma cell lines and tissues, but almost undetectable in non-melanoma cell lines or PBMC from healthy donors. Both isoforms were expressed significantly higher in melanoma tissues than in cell lines. Two of 31 melanoma cell lines expressed only one isoform or the other.ConclusionMITF-Mdel, a novel melanocyte/melanoma-specific isoform of MITF-M, may serve as a potential candidate biomarker for diagnostic and follow-up purposes in melanoma.

【 授权许可】

Unknown   
© Wang et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.